Archives of Virology

, Volume 83, Issue 3–4, pp 169–179 | Cite as

Reassortants of influenza B viruses for use in vaccines: An evaluation

  • Anne C. Goodeve
  • R. Jennings
  • C. W. Potter
Original Papers


Three slightly different procedures for the preparation of influenza B virus reassortants from B/Lee/40 and B/Lyon/79, and B/Johannesburg/58 and B/Hong Kong/82 parental viruses are described. Following cloning procedures in eggs or allantois-on-shell cultures in the presence of antisera to B/Lee or B/Johannesburg, twenty-eight putative reassortants were obtained. Using SDS-PAGE to determine the migration rates of virion polypeptides, eighteen isolates from mixed infections of B/Lee with B/Lyon, and three isolates from mixed infections of B/Johannesburg with B/Hong Kong, were found to be reassortants. All reassortants possessed surface proteins derived from either B/Lyon or B/Hong Kong and one to three internal polypeptides from either B/Lee or B/Johannesburg.

None of the reassortants showed a growth capacity in embryonated eggs as high as that of their B/Lee or B/Johannesburg parent viruses.

The procedures used, and the influenza B reassortants thus generated, would appear to offer no major advantages over the existing methods and influenza B strains at present employed for the preparation of inactivated influenza virus vaccines.


Infectious Disease Influenza Polypeptide Influenza Virus Surface Protein 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Anne C. Goodeve
    • 1
    • 2
  • R. Jennings
    • 1
  • C. W. Potter
    • 1
  1. 1.Department of VirologyThe University of Sheffield Medical SchoolSheffieldUnited Kingdom
  2. 2.Department of Molecular Biology, Paterson LaboratoriesChristie Hospital and Holt Radium InstituteWithington, ManchesterUK

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