Archives of Virology

, Volume 83, Issue 3–4, pp 169–179 | Cite as

Reassortants of influenza B viruses for use in vaccines: An evaluation

  • Anne C. Goodeve
  • R. Jennings
  • C. W. Potter
Original Papers

Summary

Three slightly different procedures for the preparation of influenza B virus reassortants from B/Lee/40 and B/Lyon/79, and B/Johannesburg/58 and B/Hong Kong/82 parental viruses are described. Following cloning procedures in eggs or allantois-on-shell cultures in the presence of antisera to B/Lee or B/Johannesburg, twenty-eight putative reassortants were obtained. Using SDS-PAGE to determine the migration rates of virion polypeptides, eighteen isolates from mixed infections of B/Lee with B/Lyon, and three isolates from mixed infections of B/Johannesburg with B/Hong Kong, were found to be reassortants. All reassortants possessed surface proteins derived from either B/Lyon or B/Hong Kong and one to three internal polypeptides from either B/Lee or B/Johannesburg.

None of the reassortants showed a growth capacity in embryonated eggs as high as that of their B/Lee or B/Johannesburg parent viruses.

The procedures used, and the influenza B reassortants thus generated, would appear to offer no major advantages over the existing methods and influenza B strains at present employed for the preparation of inactivated influenza virus vaccines.

Keywords

Infectious Disease Influenza Polypeptide Influenza Virus Surface Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Aymard-Henry, M., Coleman, M. T., Dowdle, W. R., Laver, W. G., Schild, G. C., Webster, R. G.: Influenza neuraminidase and neuraminidase-inhibition test procedures. Bull. Wld. Hlth. Org.48, 199–202 (1973).Google Scholar
  2. 2.
    Baez, M., Palese, P., Kilbourne, E. D.: Gene composition of high-yielding influenza vaccine strains obtained by recombination. J. Infect. Dis.141, 362–365 (1980).Google Scholar
  3. 3.
    Beare, A. S., Keast, K. A.: Influenza virus plaque formation in different species of cell monolayers. J. gen. Virol.22, 347–354 (1974).Google Scholar
  4. 4.
    Beare, A. S., Sherwood, J. E., Callow, K. A., Craig, J. W.: Selection of influenza B virus recombinants and their testing in humans for attenuation and immunogenicity. Infect. Immun.15, 347–353 (1977).Google Scholar
  5. 5.
    Chakraverty, P.: Antigenic relationships between influenza B viruses. Bull. Wld. Hlth. Org.45, 755–766 (1971).Google Scholar
  6. 6.
    Chakraverty, P.: Antigenic relationships between the neuraminidases of influenza B viruses. Bull. Wld. Hlth. Org.46, 473–476 (1972).Google Scholar
  7. 7.
    Davenport, F. M., Francis, T.: A comparison of the growth curves of adapted and unadapted lines of influenza virus. J. Exp. Med.89, 129–137 (1949).Google Scholar
  8. 8.
    Fazekas de St. Groth, S., White, D. O.: An improved assay for the infectivity of influenza viruses. J. Hyg.56, 151–162 (1958).Google Scholar
  9. 9.
    Ghenkina, D. B., Ghendon, Y. Z.: Recombination and complementation between orthomyxoviruses under conditions of abortive infection. Acta Virol.23, 97–106 (1979).Google Scholar
  10. 10.
    Goodeve, A. C., Potter, C. W., Clark, A., Jennings, R., Schild, G. C., Yetts, R.: A graded dose study of inactivated surface antigen influenza B vaccine in volunteers: reactogenicity, antibody response and protection to challenge virus infection. J. Hyg. (Camb.)90, 107–115 (1983).Google Scholar
  11. 11.
    Hamzawi, M., Jennings, R., Potter, C. W.: Antigenicity in hamsters of inactivated vaccines prepared from recombinant influenza viruses. J. Hyg. (Camb.)87, 453–464 (1981).Google Scholar
  12. 12.
    Henle, W., Rosenberg, E. R.: One step growth curves of various strains of influenza A and B viruses and their inhibition by inactivated virus of the homologous type. J. Exp. Med.89, 279–285 (1949).Google Scholar
  13. 13.
    Higashihara, M., Igarashi, Y., Nakagawa, S., Satio, Y.: Recombinant influenza-virus vaccines. I. Rapid derivation and preliminary characterisation of high-yielding recombinants of influenza virus. Kitasato Arch. Exp. Med.49, 1–20 (1976).Google Scholar
  14. 14.
    Kilbourne, E. D.: Future influenza vaccines and the use of genetic recombinants. Bull. Wld. Hlth. Org.41, 643–645 (1969).Google Scholar
  15. 15.
    Kilbourne, E. D., Schulman, J. L., Schild, G. C., Schloer, G., Swanson, J., Bucher, D.: Correlated studies of a recombinant influenza virus vaccine. J. Infect. Dis.124, 449–462 (1971).Google Scholar
  16. 16.
    Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature277, 680–685 (1970).Google Scholar
  17. 17.
    Lu Bao-Lan, Webster, R. G., Brown, L. E., Nerome, K.: Heterogeneity of influenza B viruses. Bull. Wld. Hlth. Org.61, 681–687 (1983).Google Scholar
  18. 18.
    Luzyanina, T., Nevedomskaya, G., Taros, L., Kudryavtseva, V., Smorodintsev, A.: Antigenic relationships between strains of influenza B virus. Acta Virol.23, 113–119 (1979).Google Scholar
  19. 19.
    McCahon, D., Schild, G. C.: Segregation of antigenic and biological characteristics during influenza virus recombination. J. gen. Virol.15, 73–77 (1972).Google Scholar
  20. 20.
    Medvedeva, T. E., Gordon, M. A., Aleksandrova, G. I., Smorodinstsev, A. A.: Experimental basis of enhancing the immunogenicity of influenza B virus by genetic recombination. Acta Virol.24, 385–390 (1980).Google Scholar
  21. 21.
    Oxford, J. S., Corcoran, T., Hugentobler, A. L.: Quantitative analysis of the protein composition of influenza A and B viruses using high resolution SDS polyacrylamide gels. J. Biol. Stand.9, 483–491 (1981).Google Scholar
  22. 22.
    Oxford, J. S., Schild, G. C., Alexandrova, G.: Electrophoretic migration rate differences of polypeptides of human influenza A viruses: Partial analysis of the genome of influenza vaccine recombinant viruses. Arch. Virol.65, 277–291 (1980).Google Scholar
  23. 23.
    Pereira, M. S., Chakraverty, P.: Influenza in the United Kingdom. J. Hyg. (Camb.)88, 501–512 (1982).Google Scholar
  24. 24.
    Schild, G. C., Oxford, J. S., Dowdle, W. R., Coleman, M., Pereira, M. S., Chakraverty, P.: Antigenic variation in current influenza A viruses: evidence for a high frequency of antigenic “drift” for the Hong Kong virus. Bull. Wld. Hlth. Org.51, 1–11 (1974).Google Scholar
  25. 25.
    Schulman, J. L., Palese, P.: Selection and identification of influenza virus recombinants of defined genetic composition. J. Virol.20, 248–254 (1976).Google Scholar
  26. 26.
    Tobita, K., Kilbourne, E. D.: Genetic recombination for antigenic markers of antigenically different strains of influenza B virus. J. Virol.13, 347–352 (1974).Google Scholar
  27. 27.
    Webster, R. G., Berton, M. T.: Analysis of antigenic drift in the haemagglutinin molecule of influenza B virus with monoclonal antibodies. J. gen. Virol.54, 243–251 (1981).Google Scholar
  28. 28.
    Wood, J. M., Schild, G. C., Newman, R. W., Seagroatt, V. A.: Application of an improved single-radial-diffusion technique for the assay of haemagglutinin antigen content of whole virus and subunit influenza vaccines. Develop. Biol. Stand.39, 193–200 (1977).Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Anne C. Goodeve
    • 1
    • 2
  • R. Jennings
    • 1
  • C. W. Potter
    • 1
  1. 1.Department of VirologyThe University of Sheffield Medical SchoolSheffieldUnited Kingdom
  2. 2.Department of Molecular Biology, Paterson LaboratoriesChristie Hospital and Holt Radium InstituteWithington, ManchesterUK

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