Archives of Virology

, Volume 135, Issue 1–2, pp 159–164 | Cite as

A field isolate of Sendai virus: its high virulence to mice and genetic divergence from prototype strains

  • T. Sakaguchi
  • K. Kiyotani
  • M. Sakaki
  • Y. Fujii
  • T. Yoshida
Brief Report


A field isolate of Sendai virus, the Hamamatsu strain, was far more virulent in mice than the prototype Z strain. The Hamamatsu strain replicated more efficiently in the mouse lung than the Z strain, causing deteriorating lung lesions. Nucleotide sequence analysis of the HN, F and M genes revealed that the Hamamatsu strain was divergent from the prototype Z, Harris and Fushimi strains.


Nucleotide Genetic Divergence Infectious Disease Sequence Analysis Nucleotide Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Blumberg BM, Colomba G, Roux L, Raju R, Dowling P, Chollet A, Kolakofsky D (1985) Sequence determination of the Sendai virus HN gene and its comparison to the influenza virus glycoproteins. Cell 41: 269–278Google Scholar
  2. 2.
    Blumberg BM, Giorgi C, Rose K, Kolakofsky D (1985) Sequence determination of the Sendai virus fusion protein gene. J Gen Virol 66: 317–331Google Scholar
  3. 3.
    Blumberg BM, Rose K, Simona MG, Roux L, Giorgi C, Kolakofsky D (1984) Analysis of the Sendai virus M gene and protein. J Virol 52: 626–663Google Scholar
  4. 4.
    Fukai K, Suzuki T (1955) On the characteristics of a newly-isolated hemagglutinating virus from mice. Med J Osaka Univ 6: 1–15Google Scholar
  5. 5.
    Hidaka Y, Kanda T, Iwasaki K, Nomoto A, Shioda T, Shibuta H (1984) Nucleotide sequence of a Sendai virus genome region covering the entire M gene and the 3′ proximal 1013 nucleotides of the F gene. Nucleic Acids Res 21: 7965–7973Google Scholar
  6. 6.
    Ishida N, Homma M (1978) Sendai virus. Adv Virus Res 23: 349–383Google Scholar
  7. 7.
    Itoh M, Shibuta H, Homma M (1987) Single amino acid substitution of Sendai virus at the cleavage site of the fusion protein confers trypsin resistance. J Gen Virol 68: 2939–2944Google Scholar
  8. 8.
    Itoh M, Tan DM, Hayashi T, Mochizuki Y, Homma M (1990) Pneumopathogenicity of a Sendai virus protease-activation mutant, TCs, which is sensitive to trypsin and chymotrypsin. J Virol 64: 5660–5664Google Scholar
  9. 9.
    Kiyotani K, Takao S, Sakaguchi T, Yoshida T (1990) Immediate protection of mice from lethal wild-type Sendai virus (HVJ) infections by a temperature sensitive mutant, HVJpi, possessing homologous interfering capacity. Virology 177:65–74Google Scholar
  10. 10.
    Kuroya M, Ishida N, Shiratori T (1953) Newborn virus pneumonitis (type Sendai) II report: the isolation of a new virus possessing hemagglutinin activity. Yokohama Med Bull 4:217–233Google Scholar
  11. 11.
    Mochizuki Y, Tashiro M, Homma M (1988) Pneumopathogenicity in mice of a Sendai virus mutant, TSrev-58, is accompanied by in vitro activation with trypsin. J Virol 62:3040–3042Google Scholar
  12. 12.
    Neubert WJ, Willenbrink W (1990) Cloning and sequencing of the HN gene of Sendai virus (strain Fushimi). Nucleic Acids Res 18:6427Google Scholar
  13. 13.
    Sakaguchi T, Toyoda T, Gotoh B, Inocencio NM, Kuma K, Miyata T, Nagai Y (1989) Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene. Virology 169: 260–272Google Scholar
  14. 14.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  15. 15.
    Shioda T, Hidaka Y, Kanda T, Shibuta H, Nomoto A, Iwasaki K (1983) Sequence of 3687 nucleotides from the 3′ end of Sendai virus genome RNA and the predicted amino acid sequences of viral NP, P and C proteins. Nucleic Acids Res 11: 7317–7330Google Scholar
  16. 16.
    Shioda T, Iwasaki K, Shibuta H (1986) Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Res 14: 1545–1563Google Scholar
  17. 17.
    Tashiro M, Homma M (1983) Pneumotropism of Sendai virus in relation to protease-mediated activation in mouse lungs. Infect Immun 39: 879–888Google Scholar
  18. 18.
    Toyoda T, Sakaguchi T, Hirota H, Gotoh B, Kuma K, Miyata T, Nagai Y (1989) Newcastle disease virus evolution. II. Lack of gene recombination in generating virulent and avirulent strains. Virology 169: 273–282Google Scholar
  19. 19.
    Van Wyke Coelingh KL, Winter CC, Murphy BR (1988) Nucleotide and deduced amino acid sequence of hemagglutinin-neuraminidase genes of human type 3 parainfluenza viruses isolated from 1957 to 1983. Virology 162: 137–143Google Scholar
  20. 20.
    Willenbrink W, Neubert WJ (1990) Cloning and sequencing of the matrix protein gene (M) of Sendai virus (strain Fushimi). Nucleic Acids Res 18: 3993Google Scholar
  21. 21.
    Yamaguchi R, Iwai H, Ueda K (1988) Variation of virulence and other properties among Sendai virus strains. Microbiol Immunol 32: 235–240Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • T. Sakaguchi
    • 1
  • K. Kiyotani
    • 1
  • M. Sakaki
    • 1
  • Y. Fujii
    • 1
  • T. Yoshida
    • 1
  1. 1.Department of BacteriologyHiroshima University School of MedicineHiroshimaJapan

Personalised recommendations