Archives of Virology

, Volume 126, Issue 1–4, pp 21–33 | Cite as

Characterization of neutralizing antibodies to bovine enterovirus elicited by synthetic peptides

  • M. S. Smyth
  • A. Trudgett
  • E. M. Hoey
  • S. J. Martin
  • F. Brown
Original Papers

Summary

Six synthetic peptides corresponding to regions of bovine enterovirus (BEV), strain VG-5-27, elicited antibodies in mice which reacted with the virus in various assays. These antibodies have been characterised on the basis of their ability to (1) neutralize the virus, (2) bind to the intact virus particle in an immunoprecipitation test, (3) react with the denatured viral proteins, and (4) give immunofluorescent staining of virus infected cells. We have also determined the proportion of antipeptide antibody which binds to the virus in each case. All of the sera immunoprecipitated the virus and neutralized its activty to varying extents. Two of the sera specific for VP 1 sequences failed to react with denatured VP 1 whereas all the other antisera reacted with their respective parental proteins. All of the sera reacted with VG-5-27 infected cells in an immunofluorescence test. The proportion of antibodies to each peptide recognizing intact virus was variable and did not appear to correlate with neutralizing activity. In addition, the ability of each of the sera to react with and neutralize three other strains of the virus was analysed. With one of these strains significant cross-neutralization was observed.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Acharya R, Fry E, Stuart D, Fox G, Rowlands D, Brown F (1989) The three dimensional structure of foot-and-mouth disease virus at 2.9 Å resolution. Nature 337: 709–716Google Scholar
  2. 2.
    Alexander H, Johnson DA, Rosen J, Jerabek L, Green N, Weismann IL, Lerner RA (1983) Mimicking the alloantigenicity of proteins with chemically synthesised peptides differing in single amino acids. Nature 306: 697–699Google Scholar
  3. 3.
    Barnett PV, Ouldridge EJ, Rowlands DJ, Brown F, Parry NR (1989) Neutralizing epitopes of type O foot-and-mouth disease virus. I. Identification and characterization of three functionally independent, conformational sites. J Gen Virol 70: 1483–1491Google Scholar
  4. 4.
    Bittle JL, Houghten RA, Alexander H, Shannock TM, Sutcliffe KG, Lerner RA, Rowlands DJ, Brown F (1982) Protection against foot-and-mouth disease virus by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature 298: 30–33Google Scholar
  5. 5.
    Boege U, Kobasha D, Onodera S, Palmenberg A, Scraba D (1991) Characterization of Mengo virus neutralization epitopes. Virology 181: 1–13Google Scholar
  6. 6.
    Chow M, Yabrov R, Bittle J, Hogle J, Baltimore D (1985) Synthetic peptides from four separate regions of poliovirus type 1 capsid protein VP 1 induce neutralizing antibodies. Proc Natl Acad Sci USA 82: 910–914Google Scholar
  7. 7.
    Colman PM, Laver WG, Varghese JN, Baker AT, Tulloch PA, Air GM, Webster PG (1987) Three dimensional structure of a complex of anitbody with influenza virus neuraminidase. Nature 326: 358–363Google Scholar
  8. 8.
    Earle JAP, Skuce RA, Fleming CS, Hoey EM, Martin SJ (1988) Complete nucleotide sequence of a bovine enterovirus. J Gen Virol 69: 253–263Google Scholar
  9. 9.
    Emini EA, Jameson BA, Wimmer E (1983) Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides. Nature 304: 699–703Google Scholar
  10. 10.
    Emini EA, Jameson BA, Wimmer E (1984) Identification of a new neutralizing antigenic site on poliovirus coat protein VP2. J Virol 52: 719–721Google Scholar
  11. 11.
    Ferguson M, Evans DMA, MaGrath EI, Minor PD, Almond JW, Shild GC (1985) Induction by synthetic peptides of broadly reactive, type specific neutralizing antibody to poliovirus type 3. Virology 143: 505–515Google Scholar
  12. 12.
    Francis MJ, Hastings GZ, Sangar DV, Clark RP, Syred A, Rowlands DJ, Brown F (1987) A synthetic peptide which elicits neutralizing antibody to human rhinovirus type 2. J Gen Virol 68: 2687–2691Google Scholar
  13. 13.
    Hoey EM, Martin SJ (1974) A possible precursor containing RNA of a bovine enterovirus: the provirion II. J Gen Virol 24: 515–524Google Scholar
  14. 14.
    Hogle JM, Chow M, Filman DJ (1985) Three dimensional structure of poliovirus at 2.9 Å resolution. Science 229: 1358–1365Google Scholar
  15. 15.
    Houghten RA (1985) General method for the rapid synthesis of large numbers of peptides: specificity of an antigen-antibody interaction at the level of individual amino acids. Proc Natl Acad Sci USA 82: 5131–5135Google Scholar
  16. 16.
    Jameson BA, Bonin J, Murray MG, Wimmer E, Kew O (1985) Peptide-induced neutralizing antibodies to poliovirus. In: Lerner R, Channock RM, Brown F (eds) Vaccines 85. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 197–198Google Scholar
  17. 17.
    Knowles NJ, Barnett ITR (1985) A serological classification of bovine enteroviruses. Arch Virol 83: 141–145Google Scholar
  18. 18.
    Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T 4. Nature 227: 680–685Google Scholar
  19. 19.
    Luo M, Vriend G, Kramer G, Minor I, Arnold E, Rossmann MG, Boege V, Scraba DG, Duke GM, Palmenberg AC (1987) The atomic structure of Mengo virus at 3.0 Å resolution. Science 235: 182–191Google Scholar
  20. 20.
    McCahon D, Crowther JR, Belsham GJ, Kitson JDA, Duchesne M, Have P, Meloen RH, Morgan DO, De Simone F (1989) Evidence for at least four antigenic sites of type O foot-and-mouth disease virus involved in neutralization; identification by single and multiple site monoclonal antibody-resistant mutants. J Gen Virol 70: 639–645Google Scholar
  21. 21.
    Martin SJ, Johnson MD, Clements JB (1970) Purification and characterization of a bovine enterovirus. J Gen Virol 7: 103–113Google Scholar
  22. 22.
    Minor PD, Schild GC, Bootman J, Evans DMA, Ferguson M, Reeve P, Spitz M, Stanway G, Cannon AJ, Hauptmann R, Clarke BLD, Mountford RC, Almond JW (1983) Location and primary structure of a major antigenic site for poliovirus neutralization. Nature 301: 674–679Google Scholar
  23. 23.
    Minor PD, Ferguson M, Evans DMA, Almond JW, Icenogle JP (1986) Antigenic structure of poliovirus of serotypes 1, 2, and 3. J Gen Virol 67: 1283–1291Google Scholar
  24. 24.
    Parry NR, Syred A, Rowlands DJ, Brown F (1988) A high proportion of anti-peptide antibodies recognize foot-and-mouth disease virus particles. Immunology 64: 567–572Google Scholar
  25. 25.
    Parry NR, Barnett PV, Ouldridge EJ, Rowlands DJ, Brown F (1989) Neutralizing epitopes of type O foot-and-mouth disease virus. II. Mapping three conformational sites with synthetic peptide reagents. J Gen Virol 70: 1493–1503Google Scholar
  26. 26.
    Pfaff E, Mussgay M, Bohm HO, Schulz GE, Schaller H (1982) Antibodies against a preselected peptide recognize and neutralize foot-and-mouth disease virus. EMBO J 1: 869–874Google Scholar
  27. 27.
    Rossmann MG, Arnold E, Erickson JW, Frankenberger EA, Griffith JP, Hecht HJ, Johnson JE, Kamer G, Luo M, Mosser AG, Rueckert RR, Sherry B, Vriend G (1985) Structure of a human common cold virus and functional relationship to other picornaviruses. Nature 317: 145–153Google Scholar
  28. 28.
    Rowlands DJ, Clarke BE, Carrol AR, Brown F, Nicholson BH, Bittle JL, Houghten RA, Lerner RA (1983) Chemical basis of antigenic variation on foot-and-mouth disease virus. Nature 306: 694–697Google Scholar
  29. 29.
    Sherry B, Rueckert RR (1985) Evidence for at least two dominant neutralization antigens on human rhinovirus 14. J Virol 53: 137–143Google Scholar
  30. 30.
    Sherry B, Mosser AG, Colonno RJ, Rueckert RR (1986) Use of monoclonal antibodies to identify four neutralization immunogens on a common cold picornavirus, human rhinovirus 14. J Virol 57: 246–257Google Scholar
  31. 31.
    Smyth MS, Hoey EM, Trudgett A, Martin SJ, Brown F (1990) Chemically synthesized peptides elicit neutralizing antibody to bovine enterovirus. J Gen Virol 71: 231–234Google Scholar
  32. 32.
    Xie Ql, McCahon D, Crowther JR, Belsham GJ, McCullough KC (1987) Neutralization of foot-and-mouth disease virus can be mediated through any of at least three separate sites. J Gen Virol 68: 1637–1647Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • M. S. Smyth
    • 1
  • A. Trudgett
    • 1
  • E. M. Hoey
    • 1
  • S. J. Martin
    • 1
  • F. Brown
    • 2
  1. 1.Medical Biology Centre, School of Biology and BiochemistryThe Queen's University of BelfastBelfast
  2. 2.Department of VirologyWellcome Biotechnology Ltd.BeckenhamU.K.

Personalised recommendations