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Archives of Virology

, Volume 127, Issue 1–4, pp 153–168 | Cite as

Identification of epitopes associated with different biological activities on the glycoprotein of vesicular stomatitis virus by use of monoclonal antibodies

  • S. Nagata
  • Y. Okamoto
  • T. Inoue
  • Y. Ueno
  • T. Kurata
  • J. Chiba
Original Papers

Summary

Thirteen monoclonal antibodies (MAbs) to the glycoprotein (G) of vesicular stomatitis virus (VSV) serotype Indiana were prepared and examined for their effects on various biological activities of VSV, including in vitro infection, hemagglutination, adsorption to cells, and mediation of cell fusion. Competitive binding assays with these MAbs revealed the presence of at least seven distinct antigenic determinants (epitopes) on the G protein. In some cases, overlappings among epitopes to various degrees were observed as partial inhibition or binding enhancement. The MAbs to all the epitopes but one (epitopes 1–6) reacted with the denatured G protein in a Western immunoblot analysis. Four of the epitopes (epitopes 2, 4, 5, and 7) were involved in neutralization and two (epitopes 1 and 2) in hemagglutination inhibition. None of the MAbs inhibited the adsorption of radiolabeled VSV to BHK-21 cells; the MAbs to epitope 2 slightly enhanced the virus adsorption. All neutralization epitopes except epitope 2 (epitopes 4, 5, and 7) were associated with inhibition of VSV-mediated cell fusion. These results show a direct spatial relationship between the epitopes recognized by the MAbs and functional sites on G protein and further insights into the structure and function of G protein.

Keywords

Monoclonal Antibody Binding Assay Immunoblot Analysis Spatial Relationship Stomatitis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • S. Nagata
    • 1
    • 2
  • Y. Okamoto
    • 1
    • 2
  • T. Inoue
    • 1
    • 2
  • Y. Ueno
    • 2
  • T. Kurata
    • 1
  • J. Chiba
    • 1
    • 3
  1. 1.Department of PathologyNational Institute of HealthTokyo
  2. 2.Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical SciencesScience University of TokyoTokyo
  3. 3.Laboratory of Immunology, Department of Biological Science and TechnologyScience University of TokyoNoda, ChibaJapan

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