Archives of Virology

, Volume 139, Issue 1–2, pp 173–181

Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection

  • E. V. Agapov
  • I. A. Razumov
  • I. V. Frolov
  • A. A. Kolykhalov
  • S. V. Netesov
  • V. B. Loktev
Brief Report
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Summary

Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • E. V. Agapov
    • 1
  • I. A. Razumov
    • 1
  • I. V. Frolov
    • 1
  • A. A. Kolykhalov
    • 1
  • S. V. Netesov
    • 1
  • V. B. Loktev
    • 1
  1. 1.Institute of Molecular BiologyState Research Center of Virology and Biotechnology "Vector"KoltsovoRussia
  2. 2.Department of Molecular MicrobiologyWashington University School of MedicineSt. LouisUSA

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