Archives of Virology

, Volume 139, Issue 1–2, pp 121–131

Biochemical and genomic characterization of muscovy duck parvovirus

  • G. Le Gall-Reculé
  • V. Jestin
Original Papers

DOI: 10.1007/BF01309459

Cite this article as:
Le Gall-Reculé, G. & Jestin, V. Archives of Virology (1994) 139: 121. doi:10.1007/BF01309459


A duck parvovirus (DPV) isolated from muscovy ducks during the epizootic in France in 1989 was purified from inoculated allanto-amniotic fluids by CsCl density gradient centrifugation and characterized. Full and empty non-enveloped icosahedral viral particles were observed banding at densities of 1.39 to 1.42 and 1.38 respectively, with a diameter of 22 to 23 nm. Viral proteins were analyzed by SDS-PAGE and the estimated molecular weights of the 3 major proteins were 91, 78 and 58 kDa. The nucleic acid was shown to be a single-stranded DNA of about 5300 bases with terminal palindromic hairpins. These results confirm the previous classification of the virus in the familyParvoviridae established by Jestin et al. [14] on morphological and serological bases. The DPV DNA was reannealed indicating that complementary DNA strands were encapsidated. A partial restriction endonuclease map was also established. This work constitutes the first biochemical and genomic description of a muscovy duck parvovirus.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • G. Le Gall-Reculé
    • 1
  • V. Jestin
    • 1
  1. 1.Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire Central de Recherches Avicole et PorcinePloufraganFrance

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