Archives of Virology

, Volume 139, Issue 1–2, pp 121–131 | Cite as

Biochemical and genomic characterization of muscovy duck parvovirus

  • G. Le Gall-Reculé
  • V. Jestin
Original Papers

Summary

A duck parvovirus (DPV) isolated from muscovy ducks during the epizootic in France in 1989 was purified from inoculated allanto-amniotic fluids by CsCl density gradient centrifugation and characterized. Full and empty non-enveloped icosahedral viral particles were observed banding at densities of 1.39 to 1.42 and 1.38 respectively, with a diameter of 22 to 23 nm. Viral proteins were analyzed by SDS-PAGE and the estimated molecular weights of the 3 major proteins were 91, 78 and 58 kDa. The nucleic acid was shown to be a single-stranded DNA of about 5300 bases with terminal palindromic hairpins. These results confirm the previous classification of the virus in the familyParvoviridae established by Jestin et al. [14] on morphological and serological bases. The DPV DNA was reannealed indicating that complementary DNA strands were encapsidated. A partial restriction endonuclease map was also established. This work constitutes the first biochemical and genomic description of a muscovy duck parvovirus.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bates RC, Snyder CE, Banerjee PT, Mitra S (1984) Autonomous parvovirus LuIII encapsidates equal amounts of plus and minus DNA strands. J Virol 49: 319–324Google Scholar
  2. 2.
    Berns KI (1990) Parvoviridae and their replication. In: Fields BN, Knipe DM (eds) Virology. Raven Press, New York pp 1743–1763Google Scholar
  3. 3.
    Berns KI, Hauswirth WW (1978) Parvovirus DNA structure and replication. In: Ward DC, Tattersall P (eds) Replication of mammalian parvoviruses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 13–32Google Scholar
  4. 4.
    Berns KI, Rose JA (1970) Evidence for a single-stranded adeno-associated virus genome: isolation and separation of complementary single strands. J Virol 5: 693–699Google Scholar
  5. 5.
    Carman PS, Povey RC (1983) Comparison of the viral proteins of canine parvovirus-2, mink enteritis and feline panleukopenia virus. Vet Microbiol 8: 423–435Google Scholar
  6. 6.
    Carter BJ, Khoury G (1975) Specific cleavage of adenovirus-associated virus DNA by restriction endonuclease R. Eco RI-characterization of cleavage products. Virology 63: 523–538Google Scholar
  7. 7.
    Diffoot N, Shull BC, Chen KC, Stout ER, Lederman M, Bates RC (1989) Identical ends are not required for the equal encapsidation of plus- and minus-strand parvovirus LuIII DNA. J Virol 63: 3180–3184Google Scholar
  8. 8.
    Fournier D (1991) Parvovirose du canard de Barbarie: approche vaccinale et recherches en cours. L'Aviculteur 521: 55–58Google Scholar
  9. 9.
    Gilat G, Meir I, Davidov K, Samberg Y (1989) Studies on Derzsy's disease in Muscovy ducks. Proceedings of the IXth Int. Congress World Veterinary Poultry Association, BrightonGoogle Scholar
  10. 10.
    Glavits R, Edesne Szabo K, Saghy E, Nemeth S, Salyi G, Ratz F, Szabo E, Meder M (1993) (Study on the parvovirus infection of muscovy ducks) A barbari kacsa parvovirus okozta betegségének vizsgalata. Magyar allatorvosok lapja 48: 212–219Google Scholar
  11. 11.
    Gough RE, Spackman D, Collins MS (1981) Isolation and characterisation of a parvovirus from goslings. Vet Rec 108: 399–400Google Scholar
  12. 12.
    Gubler U (1987) Second-strand cDNA synthesis: classical method. In: Berger SL, Kimmel AR (eds) Guide to molecular cloning techniques. Methods Enzymol 152: 325–329Google Scholar
  13. 13.
    Hoekstra J, Smit T, Van Brakel C (1973) Observations on host range and control of goose virus hepatitis. Avian Pathol 2: 169–178Google Scholar
  14. 14.
    Jestin V, Le Bras MO, Cherbonnel M, Le Gall G, Bennejean G (1991) Mise en évidence de parvovirus (virus de la maladie de Derzsy) très pathogènes dans les élevages de canards de Barbarie. Rec Med Vet 167: 849–857Google Scholar
  15. 15.
    Kisary J (1976) Buoyant density of goose parvovirus strain “B”. Acta Microbiol Hung 23: 205–207Google Scholar
  16. 16.
    Kisary J, Avalosse B, Miller-faures A, Rommelaere J (1985) The genome structure of a new chicken virus identifies it as a parvovirus. J Gen Virol 66: 2259–2263Google Scholar
  17. 17.
    Kisary J, Derzsy D (1974) Viral disease of goslings. IV. Characterisation of the causal agent in tissue culture system. Acta Vet Acad Sci Hung 24: 287–292Google Scholar
  18. 18.
    Kisary J, Miller-Faures A, Rommelaere J (1987) Presence of fowl parvovirus in fibroblast cell cultures prepared from uninoculated white Leghorn chicken embryos. Avian Pathol 16: 115–121Google Scholar
  19. 19.
    Kisary J, Nagy B, Bitay Z (1984) Presence of parvoviruses in the intestine of chickens showing stunting syndrome. Avian Pathol 13: 339–343Google Scholar
  20. 20.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685Google Scholar
  21. 21.
    Lu YS, Lin DF, Lee YL, Liao YK, Tsai HJ (1993) Infectious bill atrophy syndrome caused by parvovirus in a co-outbreak with duck viral hepatitis in ducklings in Taiwan. Avian Dis 37: 591–596Google Scholar
  22. 22.
    Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. 1st edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  23. 23.
    Mayor HD, Torikai K, Melnick JL, Mandel M (1969) Plus and minus single-stranded DNA separately encapsidated in adeno-associated satellite virions. Science 166: 1280–1282Google Scholar
  24. 24.
    Morinet F, Tratschin JD, Perol Y, Siegl G (1986) Comparison of 17 isolates of the human parvovirus B19 by restriction enzyme analysis. Arch Virol 96: 165–172Google Scholar
  25. 25.
    Saemundsen AK, Bates RC (1978) Characterization of bovine parvovirus (BPV) DNA. Virginia J Sci 29: 109Google Scholar
  26. 26.
    Salzman LA, Fabisch P, Parr R, Garon C, Wali T (1978) In vitro synthesis of double-stranded DNA from the Kilham rat virus single-stranded DNA genome. J Virol 27: 784–790Google Scholar
  27. 27.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring HarborGoogle Scholar
  28. 28.
    Summers J, Jones SE, Anderson MJ (1983) Characterization of the genome of the agent of erythrocyte aplasia permits its classification as a human parvovirus. J Virol 64: 2527–2532Google Scholar
  29. 29.
    Tattersall P (1978) Parvovirus protein structure and virion maturation. In: Ward DC, Tattersall P (eds) Replication of mammalian parvoviruses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, pp 53–72Google Scholar
  30. 30.
    Tullis GE, Burger LR, Pintel DJ (1993) The minor capsid protein VP1 of the autonomous parvovirus minute virus of mice is dispensable for encapsidation of progeny single-stranded DNA but is required for infectivity. J Virol 67: 131–141Google Scholar
  31. 31.
    Ziedler K, Peter W, Sobanski E (1984) (Studies of the agent of enterohepatitis of Muscovy ducks) Untersuchungen zum Erreger der enterohepatitis der Cairina. Monatshefte für Veterinärmedizin 39: 374–377Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • G. Le Gall-Reculé
    • 1
  • V. Jestin
    • 1
  1. 1.Centre National d'Etudes Vétérinaires et Alimentaires, Laboratoire Central de Recherches Avicole et PorcinePloufraganFrance

Personalised recommendations