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Archives of Virology

, Volume 128, Issue 3–4, pp 363–370 | Cite as

Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity

  • M. S. Collins
  • J. B. Bashiruddin
  • D. J. Alexander
Brief Report

Summary

The amino acid sequence at the F2/F1 cleavage site of the F0 fusion protein of 17 strains of Newcastle disease virus (NDV) was deduced from sequencing a 32 nucleotide area of the genome by reverse transcription and polymerase chain reaction (PCR) techniques. With the addition of sequences at the same area previously published for 9 other viruses comparisons were made of a total of 26 NDV strains and isolates (11 of low virulence, 15 of high virulence or mesogenic) covering ten antigenic groups determined by reactions with monoclonal antibodies. All the virulent viruses and the mesogenic strain Komarov showed the amino acid sequence112R/K-R-Q-K/R-R116 for the C-terminus of the F2 protein and phenylalanine (F) at the N-terminus of the F1 protein, residue 117. The mesogenic isolate of the antigenic variant NDV responsible for the recent panzootic in racing pigeons, often termed “pigeon paramyxovirus type 1”, examined in this study had the sequence112G-R-Q-K-R-F117. The deduced amino acid sequence in the corresponding region of all viruses of low virulence was112G/E-K/R-Q-G/E-R-L117.

The virulent virus, PMV-1/chicken/Ireland/34/90 (34/90), which had a close antigenic relationship to a group of avirulent viruses, three of which were examined in the present study as representatives of the monoclonal antibody group H, showed between 4–6 nucleotide differences from these viruses in the 32 nucleotide region studied. These resulted in differences in the deduced amino acid sequence at residue 112 E → K, 115 E → K and 117 → F, giving 34/90 a typical virulent virus motif at the cleavage site. Despite the extremely small portion of the genome studied there were several areas which appeared characteristic for 34/90 and the three group H viruses of low virulence, which suggests that they may have arisen from the same gene pool.

Keywords

Cleavage Site Deduce Amino Acid Sequence Newcastle Disease Virus Virulent Virus Newcastle Disease Virus Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Alexander DJ (1990) Avian Paramyxoviridae — recent developments. Vet Microbiol 23: 103–114Google Scholar
  2. 2.
    Alexander DJ, Campbell G, Manvell RJ, Collins MS, Parsons G, McNulty MS (1992) Characterisation of an antigenically unusual virus responsible for two outbreaks of Newcastle disease in the Republic of Ireland in 1990. Vet Rec 130: 65–68Google Scholar
  3. 3.
    Alexander DJ, Parsons G (1986) Pathogenicity for chickens of avian paramyxovirus type 1 isolates obtained from pigeons in Great Britain during 1983–1985. Avian Pathol 15: 487–493Google Scholar
  4. 4.
    Alexander DJ, Parsons G, Manvell RJ, Sayers AR (1992) Avian paramyxovirus type 1 infections of racing pigeons in Great Britain — epizootiological and laboratory aspects of virus isolates. In: Proceedings of the British Veterinary Poultry Association Seminar on Diseases of Pigeons (in press)Google Scholar
  5. 5.
    Della-Porta AJ, Hansson E, Russell G, Young J, Bashiruddin J, Gould AR, Darminto H, Hamid H, Daniels P (1992) The use of markers of virulence to develop rapid pathotyping assays for Newcastle disease virus. In: Proceedings of the Negative Strand Virus Conference, Charleston 1991 (in press)Google Scholar
  6. 6.
    Glickman RL, Syddall RJ, Iorio RM, Sheenan JP, Bratt MA (1988) Quantitative basic residue requirements in the cleavage-activation site of the fusion glycoprotein as a determinant of virulence for Newcastle disease virus. J Virol 62: 354–356Google Scholar
  7. 7.
    Gotoh B, Sakaguchi T, Nishikawa K, Inocencio NM, Hamaguchi M, Toyoda T, Nagai Y (1988) Structural features unique to each of the three antigenic sites on the hemagglutinin-neuraminidase protein of Newcastle disease virus. Virology 163: 174–182Google Scholar
  8. 8.
    Hosaka M, Nagahama N, Kim WS, Watanabe T, Hatsuzama K, Ikemizu J, Murakami K, Nakayama K (1991) Arg-X-lys/arg-arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway. J Biol Chem 266: 12127–12130Google Scholar
  9. 9.
    Millar NS, Chambers P, Emmerson PT (1988) Nucleotide sequence of the fusion and haemagglutinin-neuraminidase glycoprotein genes of Newcastle disease virus, strain Ulster: molecular basis for variations in pathogenicity between strains. J Gen Virol 69: 613–620Google Scholar
  10. 10.
    Nagai Y, Hamaguchi M, Toyoda T (1889) Molecular biology of Newcastle disease virus. Prog Vet Microbiol Immunol 5: 16–64Google Scholar
  11. 11.
    Pritzer E, Kuroda K, Garten W, Nagai Y, Klenk H-D (1990) A host range mutant of Newcastle disease virus with an altered cleavage site for proteolytic activation of the F protein. Virus Res 15: 237–242Google Scholar
  12. 12.
    Rott R, Klenk H-D (1988) Molecular basis of infectivity and pathogenicity of Newcastle disease virus. In: Alexander DJ (ed) Newcastle disease. Kluwer, Norwell, pp 98–112Google Scholar
  13. 13.
    Russell PH, Alexander DJ (1983) Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodies. Arch Virol 75: 243–253Google Scholar
  14. 14.
    Russell PH, Samson ACR, Alexander DJ (1990) Newcastle disease virus variations. Appl Virus Res 2: 177–195Google Scholar
  15. 15.
    Sakaguchi T, Matsuda Y, Kiyokage R, Kawahara N, Kiyotani K, Katunuma N, Nagai Y, Yoshida T (1991) Identification of endoprotease activity in thetrans Golgi membranes of rat liver cells that specifically processes in vitro the fusion glycoprotein precursor of virulent Newcastle disease virus. Virology 184: 504–512Google Scholar
  16. 16.
    Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, a laboratory manual, 2nd edn. Cold Spring Harbour Press, New YorkGoogle Scholar
  17. 17.
    Schaper UM, Fuller FJ, Ward MDW, Mehrotra Y, Stone HO, Stripp BR, De Buysscher E (1988) Nucleotide sequence of the envelope protein genes of a highly virulent, neurotropic strain of Newcastle disease virus. Virology 165: 291–295Google Scholar
  18. 18.
    Schuy W, Garten W, Lindner D, Klenk H-D (1984) The caboxyterminus of the haemagglutinin-neuraminidase of Newcastle disease virus is exposed at the surface of the viral envelope. Virus Res 1: 415–426Google Scholar
  19. 19.
    Toyoda T, Sakaguchi T, Hirota H, Gotoh B, Kuma K, Miyata T, Nagai Y (1989) Newcastle disease evolution II Lack of gene recombination in generating virulent and avirulent strains. Virology 169: 273–282Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. S. Collins
    • 1
  • J. B. Bashiruddin
    • 1
  • D. J. Alexander
    • 1
  1. 1.Avian VirologyCentral Veterinary LaboratoryWeybridgeUK

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