Archives of Virology

, Volume 77, Issue 2–4, pp 223–229 | Cite as

Determination of ribonucleoside triphosphate pools in influenza A virus-infected MDCK cells

  • S. Stridh
Original Papers


A rapid method for determining the concentration of ribonucleoside triphosphates in cell cultures is described. The pool sizes of the ribonucleoside triphosphates in uninfected MDCK cells were found to be, 0.28–0.59mm CTP, 0.96–1.6mm UTP, 6.5–7.8mm ATP, and 1.1–1.4mm GTP, depending on the condition of the cell-monolayer. Infection of MDCK cells with influenza A strains Victoria 3/75 x-47 or WSN resulted in insignificant changes in the ribonucleoside triphosphate concentrations. Treatment of infected and non-infected MDCK cells with ribavirin caused a decrease of 60 per cent in the GTP pool.


Cell Culture Infectious Disease Influenza Triphosphate Rapid Method 
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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. Stridh
    • 1
  1. 1.Department of Antiviral Chemotherapy, Research and Development LaboratoriesAstra Läkemedel ABSödertäljeSweden

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