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Archives of Virology

, Volume 77, Issue 2–4, pp 223–229 | Cite as

Determination of ribonucleoside triphosphate pools in influenza A virus-infected MDCK cells

  • S. Stridh
Original Papers

Summary

A rapid method for determining the concentration of ribonucleoside triphosphates in cell cultures is described. The pool sizes of the ribonucleoside triphosphates in uninfected MDCK cells were found to be, 0.28–0.59mm CTP, 0.96–1.6mm UTP, 6.5–7.8mm ATP, and 1.1–1.4mm GTP, depending on the condition of the cell-monolayer. Infection of MDCK cells with influenza A strains Victoria 3/75 x-47 or WSN resulted in insignificant changes in the ribonucleoside triphosphate concentrations. Treatment of infected and non-infected MDCK cells with ribavirin caused a decrease of 60 per cent in the GTP pool.

Keywords

Cell Culture Infectious Disease Influenza Triphosphate Rapid Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Brenton, D. P., Astin, K. H., Cruikshank, M. K., Seegmiller, J. E.: Measurement of free nucleotides in cultured human lymphoid cells using high pressure liquid chromatography. Biochem. Med.17, 231 (1977).Google Scholar
  2. 2.
    Caric-Lazar, M., Schwarz, R. T., Scholtissek, C.: Influence of the infection with lipid-containing viruses on the metabolism and pools of phospholipid precursors in animal cells. Eur. J. Biochem.91, 351–361 (1978).Google Scholar
  3. 3.
    Choppin, P. W.: Replication of influenza virus in a continuous cell line: High yield of infective virus from cells inoculated of high multiplicity. Virology39, 130–134 (1969).Google Scholar
  4. 4.
    Dämmgen, J. W., Scholtissek, C.: Cellular RNA and influenza-virion RNA are synthesized from different pyrimidine-nucleoside-triphosphate pools in chickembryo cells. Eur. J. Biochem.59, 51–54 (1975).Google Scholar
  5. 5.
    Garret, C., Santi, D. V.: A rapid and sensitive high pressure liquid chromatography assay for deoxyribonucleoside triphosphates in cell extracts. Anal. Biochem.99, 268–273 (1979).Google Scholar
  6. 6.
    Gudas, L. J., Cohen, A., Ullman, B., Martin, D. W., jr.: Analysis of adenosine-mediated pyrimidine starvation using cultured wild-type and mutant mouse T-lymphoma cells. Somatic Cell Genetics4, 201–219 (1978).Google Scholar
  7. 7.
    Hammer, G., Schwarz, R. T., Scholtissek, C.: Effect of infection with enveloped viruses on nucleotide metabolism. Virology70, 238–240 (1976).Google Scholar
  8. 8.
    Jamieson, A. T., Bjursell, G.: Deoxyribonucleoside triphosphate pools in herpes simplex virus type 1 infected cells. J. gen. Virol.31, 101–113 (1976).Google Scholar
  9. 9.
    Scholtissek, C., Rott, R., Klenk, H.-D.: Two different mechanism of the inhibition of the multiplication of enveloped viruses by glucosamine. Virology63, 191–200 (1975).Google Scholar
  10. 10.
    Scholtissek, C.: Inhibition of influenza RNA synthesis by Virazole (Ribavirin). Arch. Virol.50, 349–352 (1976).Google Scholar
  11. 11.
    Skovsgaard, T.: Transport and Binding of Daunorubicin, Adriamycin, and Rubidazone in Ehrlich Ascites Tumour Cells. Biochem. Pharmacol.26, 215–222 (1977).Google Scholar
  12. 12.
    Smith, R., Kirkpatrik, W.: Ribavirin. A Broad Spectrum Antiviral Agent. Academic Press 1980.Google Scholar
  13. 13.
    Stollar, V., Malinoski, F.: The effects on adenosine and guanosine on the replication of Sindbis and vesicular stomatitis viruses in Aedes albopictus cells. Virology115, 57–66 (1981).Google Scholar
  14. 14.
    Stridh, S., Öberg, B., Chattophadhyaya, J., Josephson, S.: Functional analysis of influenza RNA polymerase activity by the use of caps, oligonucleotides and polynucleotides. Antiviral Res.1, 97–105 (1981).Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. Stridh
    • 1
  1. 1.Department of Antiviral Chemotherapy, Research and Development LaboratoriesAstra Läkemedel ABSödertäljeSweden

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