Planta

, Volume 82, Issue 2, pp 123–144 | Cite as

Auxin movement in corn coleoptiles

  • Rainer Hertel
  • Rand Flory
Article
Received:

Summary

Movement of radioactive auxins was analysed in corn coleoptile sections. The results support the idea that processes involved in the transport of indoleacetic acid (IAA) are specific for growth-promoting auxins.

Inhibition of IAA transport by triiodobenzoic acid is caused by a reversible block of the exit; the auxin held back remains in the transport pool. The observed increase in immobilization may be a secondary effect caused by the increased concentration of free IAA in the tissue.

Auxin molecules are most likely transported by anon-covalent mechanism. IAA and naphthaleneacetic acid (NAA) move through the cell and exit as free molecules. A search for a transient auxin complex, chaseable as required for any transport carrier intermediate, yielded negative results. No18O was lost from NAA labeled with18O in the carboxyl group during transport of the auxin through coleoptile tissue.

After application of IAA to auxin-depleted tissue, the transport rate undergoes oscillations with a period length of ca. 25 min.

The movement of the auxin 2.4-dichlorophenoxyacetic acid which is usually sluggish, increased several times if some IAA was added. Auxin, thus, stimulates its own transport.

A model is discussed in which auxin-binding to the plasma membrane and reversible changes of membrane conformation may provide a basis for active secretion and for the observed cooperativity.

Keywords

Immobilization Active Secretion Transport Carrier Indoleacetic Acid Period Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • Rainer Hertel
    • 1
  • Rand Flory
    • 1
  1. 1.MSU/AEC Plant Research LaboratoryMichigan State UniversityEast Lansing

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