Digestive Diseases and Sciences

, Volume 37, Issue 11, pp 1725–1728 | Cite as

Phenotypic characterization of isolated intraepithelial lymphocytes in patients with ulcerative colitis and normal controls

  • P. Hoang
  • M. Senju
  • J. R. Lowes
  • D. P. Jewell
Original Articles
Received:
Revised:
Accepted:

Abstract

A method was developed to isolate colonic intraepithelial lymphocytes in patients with ulcerative colitis (N=8) and normal controls (N=13) in order to characterize their phenotype using a panel of monoclonal antibodies and flow cytometry. In both groups, the majority of the cells are of the CD3+CD8+ phenotype associated with cytotoxic/suppressor function. The CD4/CD8 ratios were similar. Virtually no B cells or macrophages were found. An increase in cells coexpressing the CD3 and HLA-DR molecules and a decrease in natural killer cells (CD3, CD16+CD56+) were found in ulcerative colitis, but this was not significant. There were no differences in the proportions of CD8+ and CD4+ cells expressing the Leu8 antigen between ulcerative colitis and controls. Thus a population of colonic intraepithelial lymphocytes has been isolated and, although they may be functionally different in ulcerative colitis compared with controls, this cannot be explained in terms of phenotypic characteristics.

Key Words

mucosal lymphocytes flow cytometry 
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Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • P. Hoang
    • 1
  • M. Senju
    • 1
  • J. R. Lowes
    • 1
  • D. P. Jewell
    • 1
  1. 1.Gastroenterology UnitRadcliffe InfirmaryOxfordUK

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