Summary
The tropical lianaAncistrocladus heyneanus, which is known for its biologically active naphthylisoquinoline alkaloids, has been studied by nuclear magnetic resonance (NMR) microscopy for the first time. The spatial resolution of the cross-sectional NMR images was of the order of 20 μm. Quantitative NMR relaxation time images of the root and the shoot show great contrast between different tissue regions. In addition, we observed the regional distribution of chemical compounds inAncistrocladus heyneanus by chemical-shift NMR microscopy. The NMR imaging results were compared with light and fluorescence microscopic images and reveal the excellent tissue characterization using NMR technology.
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Abbreviations
- NMR:
-
nuclear magnetic resonance
- CSI:
-
chemical-shift magnetic resonance imaging
- FOV:
-
field of view
- TE:
-
echo time
- TR:
-
repetition time
References
Bentrup FW (1996) NMR-microscopy: observing xylem and phloem conduits at work. Bot Acta 109: 177–179
Bottomley PA, Rogers HH, Foster TH (1986) NMR imaging shows water distribution and transport in plant root systems in situ. Proc Natl Acad Sci USA 83: 87–89
Bringmann G, Pokorny F (1995) The naphthylisoqumoline alkaloids. In: Cordell GA (ed) The alkaloids, vol 46. Academic Press, New York, pp 127–271
— —, Zinsmeister HD (1991)Ancistrocladus, eine botanisch und chemisch bemerkenswerte Gattung. Palmengarten 55/3: 13–18
—, Schneider C, Pokorny F, Lorenz H, Fleischmann H, Sankara Narayanan AS, Almeida MR, Govindachari TR, Aké Assi L (1993) The cultivation of tropical lianas of the genus Ancistrocladus. Planta Med 59 Suppl: 623–624
—, Koppler D, Wiesen B, Francois G, Sankara Narayanan AS, Almeida MR, Schneider H, Zimmermann U (1996) Ancistroheynine A, the first 7,8′-coupled naphthylisoqumoline alkaloid fromAncistrocladus heyneanus. Phytochemistry 43: 1405–1410
Brown TR, Kincaid BM, Ugurbil K (1982) NMR chemical shift imaging in three dimensions. Proc Natl Acad Sci USA 79: 3523–3526
Callaghan PT (1991) Principles of nuclear magnetic resonance microscopy. Clarendon Press, Oxford
Connelly A, Lohman AB, Loughman BC, Quiquampoix H, Ratcliffe RG (1987) High resolution imaging of plant tissues by NMR. J Exp Bot 38: 1713–1723
Freeman R, Hill K (1971) Fourier transform study of NMR spin-lattice relaxation by progressive saturation. J Chem Phys 54: 3367–3371
Haase A, Brandl M, Kuchenbrod E, Link A (1993) Magnetization prepared NMR microscopy. J Magn Reson A 105: 230–233
Hills BP, Duce SL (1990) The influence of chemical and diffusive exchange on water proton transverse relaxation in plant tissues. Magn Reson Imag 8: 321–331
Johnson GA, Brown J, Kramer PJ (1987) Magnetic resonance microscopy of changes in water content in stems of transpiring plants. Proc Natl Acad Sci USA 84: 2752–2755
Kuchenbrod E, Benkert R, Schneider H, Haase A, Zimmermann U (1995) Quantitative NMR microscopy on intact plants. Magn Reson Imag 13: 447–455
—, Landeck M, Thürmer F, Haase A, Zimmermann U (1996) Measurements of water flow in the xylem vessels of intact maize plants using flow-sensitive NMR imaging. Bot Acta 109: 184–186
- Kahler E, Thürmer F, Deichmann R, Zimmermann U, Haase A (1997) Functional magnetic resonance imaging in intact plants — quantitative observation of flow in plant vessels. Magn Reson Imag (in press)
MacFall JS, Johnson GA, Kramer PJ (1990) Observation of a water-depletion region surrounding loblolly pine roots by magnetic resonance imaging. Proc Natl Acad Sci USA 87: 1203–1207
—, Pfeffer PE, Rolin DB, MacFall JR, Johnson GA (1992) Observation of the oxygen diffusion barrier in soybean (Glycine max) nodules with magnetic resonance microscopy. Proc Natl Acad Sci USA 87: 1203–1207
Metzler A, Köckenberger W, von Kienlin M, Komor E, Haase A (1994) Quantitative measurement of sucrose distribution inRicinus communis seedlings by chemical-shift microscopy. J Magn Reson B 105: 249–252
—, Izquierdo M, Ziegler A, Köckenberger W, Komor E, von Kienlin M, Haase A, Décorps M (1995) Plant histochemistry by correlation peak imaging. Proc Natl Acad Sci USA 92: 11912–11915
Rumpel H, Pope J (1992) The application of 3D chemical shift microscopy to noninvasive histochemistry. Magn Reson Imag 10: 187–194
Sarafis V, Rumpel H, Pope J, Kuhn W (1990) Noninvasive histochemistry of plant materials by magnetic resonance microscopy. Protoplasma 159: 70–73
Schafsma TJ, van As H, Palstra WD, Snaar JE, de Jager PA (1992) Quantitative measurement and imaging of transport processes in plants and porous media by1H NMR. Magn Reson Imag 10: 827–836
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Meininger, M., Stowasser, R., Jakob, P.M. et al. Nuclear magnetic resonance microscopy ofAncistrocladus heyneanus . Protoplasma 198, 210–217 (1997). https://doi.org/10.1007/BF01287570
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DOI: https://doi.org/10.1007/BF01287570