Protoplasma

, Volume 213, Issue 3, pp 184–193

Actin cytoskeleton is responsible for the change of cytoplasmic organization in root hair cells induced by a protein phosphatase inhibitor, calyculin A

  • E. Yokota
  • N. Imamichi
  • M. Tominaga
  • T. Shimmen
Original Papers

DOI: 10.1007/BF01282156

Cite this article as:
Yokota, E., Imamichi, N., Tominaga, M. et al. Protoplasma (2000) 213: 184. doi:10.1007/BF01282156

Summary

In root hair cells ofLimnobium stoloniferum, a protein phosphatase inhibitor, calyculin A (CA), at concentrations higher than 50 nM inhibits cytoplasmic streaming and induces remarkable morphological changes in the cytoplasm: the transvacuolar strands disperse and spherical cytoplasmic bodies emerge. The mechanism of the morphological changes of the cytoplasm induced by CA was studied by pharmacological analyses. The formation of spherical bodies in cells treated with CA was suppressed by the actin-depolymerizing and -fragmenting drugs latrunculin B and cytochalasin D at concentrations higher than 100 nM and 5 μM, respectively. In contrast, 100 μM propyzamide, a microtubule-depolymerizing drug, did not affect the formation of spherical bodies by CA. Interestingly, 60 mM 2,3-butanedione monoxime, an inhibitor of myosin, also suppressed the CA-induced formation of cytoplasmic spherical bodies. These results indicate that the actin cytoskeleton is intimately involved in the morphological changes of the cytoplasm induced by CA.

Keywords

Actin Calyculin A Phosphatases Transvacuolar strand Cytoplasmic streaming Root hair cell 

Abbreviations

APW

artificial pond water

BDM

2,3-butanedione monoxime

CD

cytochalasin D

DMSO

dimethylsulfoxide

LB

latrunculin B

Pro

propyzamide

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • E. Yokota
    • 1
  • N. Imamichi
    • 1
  • M. Tominaga
    • 1
  • T. Shimmen
    • 1
  1. 1.Department of Life Science, Faculty of ScienceHimeji Institute of TechnologyHyogoJapan

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