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Protoplasma

, Volume 191, Issue 3–4, pp 172–177 | Cite as

Microspectrofluorometry of the autofluorescent flagellum in phototactic brown algal zoids

  • Hiroshi Kawai
  • Shago Nakamura
  • Mamoru Mimuro
  • Masaki Furuya
  • Masakatsu Watanabe
Article

Summary

Posterior flagella of zoids ofScytosiphon lomemaria andChorda filum (Phaeophyceae, Chromophyta) were isolated and subjected to microspectrofluorometry using a high sensitivity microspectrofluorometer in order to characterize the flagellar autofluorescent substances. Vigorous agitation in a Hypertonic medium yielded preparations of largely intact flagella retaining detectable green flagellar autofluorescence. Under 380–425 nm excitation light, maximum emission of flagellar autofluorescence was observed at 495 nm, whereas under 400–440 nm excitation light fluorescence shifted to around 510 nm. Comparison of these spectra with the fluorescence spectra of 4′,5′-cyclic FMN isolated from fertile thalli ofS. lomentaria, and of 6-carboxypterin suggested that two (or more) different fluorescent substances (presumably a flavin and a pterin) are present in the flagella.

Keywords

Chorda filum Flagellar fluorescence Flavin Microspectrofluorometry Phototaxis Scytosiphon lomentaria 

Abbreviations

DTT

dithiothreitol

FMN

flavin mononucleotide

HEPES

N-[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid])

PEG

polyethylene glycol

PFB

paraflagellar body

Tris

tris(hydroxymethyl) aminomethane.

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Hiroshi Kawai
    • 1
  • Shago Nakamura
    • 2
  • Mamoru Mimuro
    • 3
  • Masaki Furuya
    • 4
  • Masakatsu Watanabe
    • 3
  1. 1.Kobe University Research Center for Inland SeasKobeJapan
  2. 2.Department of Environmental Biology and Chemistry, Faculty of ScienceToyama UniversityToyama
  3. 3.National Institute for Basic BiologyOkazaki
  4. 4.Advanced Research LaboratoryHitachi Ltd.Saitama

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