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Protoplasma

, Volume 137, Issue 1, pp 56–62 | Cite as

Cytoskeletal elements in cotton seed hair developmentin vitro: Their possible regulatory role in cell wall organization

  • H. Quader
  • W. Herth
  • U. Ryser
  • E. Schnepf
Article

Summary

The localization and orientation of cytoskeletal elements in developing cotton fibres were studied by the indirect immunofluorescence and the dry cleaving technique. Microtubules are transversely arranged to the cell axis, most probably in a flat helix, in the cortex of expanding fibres. Since the innermost deposited cellulose microfibrils always show primarily the same orientation it is postulated that the microtubules control the transverse deposition of the cellulose fibrils. Little further cell expansion takes place during secondary wall formation and the microfibril pattern corresponds to that of the cortical microtubules,e.g., in the steepness of their helicoidal turns. Microtubules with a length of 7–20 μm were observed, probably they are longer. The importance of microtubule length on microfibril deposition is discussed. The density of microtubule packing is in the range of 8–14 μm-1 as in other comparable cell types. In contrast to the microtubules, actin filaments are most likely longitudinally oriented during different phases of fibre development. The dry cleaving technique reveals numerous coated pits in the plasma membrane which are not crossed by microtubules. They seem to be linked to the latter by filamentous structures.

Keywords

Cytoskeleton Cellulose fibrils Cotton fibres Coated pits 

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References

  1. Frey-Wyssling A (1976) The plant cell wall. In: Encyclopedia of plant anatomy, vol 3. Gebr. Bornträger, Berlin StuttgartGoogle Scholar
  2. Gunning BES, Hardham AR (1982) Microtubules. Ann Rev Plant Physiol 33: 651–698Google Scholar
  3. Hardham AR, Gunning BES (1978) Structure of cortical microtubule arrays in plant cells. J Cell Biol 77: 14–34Google Scholar
  4. Heath IB, Seagull RW (1982) Oriented cellulose fibrils and the cytoskeleton: A critical comparison of models. In:Lloyd CW (ed) The cytoskeleton in plant growth and development. Academic Press, London, pp 163–182Google Scholar
  5. Herth W (1985) Plant cell wall formation. In:Robards AW (ed) Botanical microscopy 1985. Oxford University Press, OxfordGoogle Scholar
  6. Itoh T (1974) Fine structure and formation of cell wall of developing cotton fiber. Wood Res 56: 49–61Google Scholar
  7. Joachim S, Robinson DG (1984) Endocytosis of cationic ferritin by bean leaf protoplasts. Eur J Cell Biol 34: 212–216Google Scholar
  8. Lloyd CW (1984) Toward a dynamic helical model for the influence of microtubules on wall patterns in plants. Int Rev Cytol 86: 1–51Google Scholar
  9. —,Wells B (1985) Microtubules are at the tips of root hairs and form helical patterns corresponding to inner wall fibrils. J Cell Sci 75: 225–238Google Scholar
  10. Meinert MC, Delmer DP (1977) Changes in biochemical composition of the cell wall of the cotton fiber during development. Plant Physiol 59: 1088–1097Google Scholar
  11. Quader H (1986) Cellulose microfibril orientation inOocystis solitaria: proof that microtubules control the alignment of the terminal complexes. J Cell Sci 83: 223–234Google Scholar
  12. — (1983) Morphology and movement of cellulose synthesizing (terminal) complexes inOocystis solitaria: evidence that microfibril assembly is the motive force. Eur J Cell Biol 32: 174–177Google Scholar
  13. —,Deichgräber G, Schnepf E (1986) The cytoskeleton ofCobaea seed hairs: patterning during cell wall differentiation. Planta 168: 1–10Google Scholar
  14. Robinson DG, Quader H (1982) The microtubule-micro fibrilsyndrome. In:Lloyd CW (ed) The cytoskeleton in plant growth and development. Academic Press, London, pp 109–126Google Scholar
  15. Roelofson PA (1951) Orientation of cellulose fibrils in the cell wall of growing cotton hairs and its bearing on the physiology of cell wall growth. Biochim Biophys Acta 7: 45–53Google Scholar
  16. Ryser U (1979) Cotton fiber differentiation: Occurence and distribution of coated and smooth vesicles during primary wall formation. Protoplasma 98: 223–239Google Scholar
  17. — (1985) Cell wall biosynthesis in differentiating cotton fibres. Eur J Cell Biol 39: 236–256Google Scholar
  18. Schnepf E, Roederer G, Herth W (1975) The formation of the fibrils in the lorica ofPoterioochromonas stipitata: Tip growth, kinetics, site, orientation. Planta 125: 45–62Google Scholar
  19. — (1974) Microtubules and cell wall formation. Portugaliae Acta Biologica 14: No 1–2, 451–462Google Scholar
  20. Seagull RW (1985) The effects of colchicine and taxol on microtubule arrays and cell wall deposition in developing cotton fibres: An immunofluorescence study. In:Robards AW (ed) Botanical microscopy 1985. Oxford University Press, OxfordGoogle Scholar
  21. —,Heath IB (1980) The organization of cortical microtubule arrays in the radish root hair. Protoplasma 103: 212–218Google Scholar
  22. —— (1979) The effects of tannic acid on thein vivo preservation of microfilaments. Eur J Cell Biol 20: 184–188Google Scholar
  23. Simmonds D, Setterfield G, Brown DL (1983) Organization of microtubules in dividing and elongating cells ofVicia hajastana Gorsh. in suspension culture. Eur J Cell Biol 32: 59–66Google Scholar
  24. Tanchak MA, Griffing CR, Mersey BG, Fowke LC (1984) Endocytosis of cationized ferritin by coated vesicles of soybean protoplasts. Planta 162: 481–486Google Scholar
  25. Traas JA (1984) Visualization of the membrane bound cytoskeleton and coated pits of plant cells by means of dry cleaving. Protoplasma 119: 212–218Google Scholar
  26. —,Braat P, Emons AMC, Meekes H, Derksen J (1985) Microtubules in root hairs. J Cell Sci 76: 303–320Google Scholar
  27. Wiche G (1985) High-molecular-weight microtubule associated proteins (MAPs): a ubiquitous family of cytoskeletal connecting links. Trends Biochem Sci 10: 67–70Google Scholar
  28. Wick SM, Seagull RW, Osborn M, Weber K, Gunning BES (1981) Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J Cell Biol 89: 685–690Google Scholar
  29. Wulf E, Deboben A, Bautz FA, Faulstich H, Wieland Th (1979) Fluorescent phallotoxin, a tool for the visualization of cellular actin. Proc Nat Acad Sci USA 76: 4498–4502Google Scholar
  30. Yatsu LY (1983) Morphological and physical effects of colchicine treatment on cotton (Gossypium hirsutum) fibre. Textile Res 53: 515–519Google Scholar
  31. —,Jacks TJ (1981) An ultrastructural study of the relationship between microtubules and microfibrils in cotton (Gossypium hirsutum L.) cell wall reversals. Am J Bot 68: 771–777Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • H. Quader
    • 1
  • W. Herth
    • 1
  • U. Ryser
    • 2
  • E. Schnepf
    • 1
  1. 1.ZellenlehreUniversität HeidelbergHeidelbergGermany
  2. 2.Institut für Biologie und PhytochemieUniversität FreiburgSwitzerland

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