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Protoplasma

, Volume 123, Issue 3, pp 184–191 | Cite as

An investigation of the role of transcellular ion currents in morphogenesis ofGriffithsia pacifica Kylin

  • Susan D. Waaland
  • William J. Lucas
Article

Summary

Transcellular ion currents are thought to play a role in the induction and maintenance of localized growth in plant cells. In the marine red algaGriffithsia pacifica, two types of cells elongate by localized tip growth, rhizoidal and repair shoot cells. The pattern of growth and morphogenesis in these cells can be altered by environmental and hormonal parameters. We examined the role of localized currents in four developmental processes inG. pacifica: 1. normal elongation of rhizoids, 2. the phototropic response of rhizoids, 3. the re-initiation of growth in dark-starved rhizoids, and 4. morphogenesis of repair shoot cells in the presence and absence of rhodomorphin, an endogenous hormone which regulates growth of these cells.

We have found that there is a localized region of inflowing current at the growing tips of both rhizoids and repair shoot cells. The current density at these apices, measured approx. 20 μm from the cell surface, fluctuates in the range of 0.6 to 8 μA cm−2 with occasional periods of either very large current (> 20 μA cm−2) or no measurable current; however, the current density is not correlated with the rate of elongation. In addition, currents of similar magnitudes are found at the tips of non-growing cells. Rhizoids which have lost their cytoplasmic polarity and have stopped elongating, following prolonged periods in total darkness, can reestablish a polar distribution of organelles and restart localized growth in the absence of any measurable current at their tips. Thus, it appears that inG. pacifica localized transcellular currents are neither sufficient or necessary for the maintenance or reinitation of sites of localized growth and organelle accumulation.

Keywords

Transcellular currents Tip growth-morphogenesis Griffithsia Red alga Vibrating probe 

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Susan D. Waaland
    • 1
  • William J. Lucas
    • 2
  1. 1.Department of BotanyUniversity of WashingtonSeattle
  2. 2.Department of BotanyUniversity of CaliforniaDavisUSA

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