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Protoplasma

, Volume 201, Issue 1–2, pp 45–52 | Cite as

Discovery of signaling effect of UV-B/C light in the extended UV-A/blue-type action spectra for step-down and step-up photophobic responses in the unicellular flagellate algaEuglena gracilis

  • S. Matsunaga
  • T. Hori
  • T. Takahashi
  • M. Kubota
  • M. Watanabe
  • K. Okamoto
  • K. Masuda
  • M. Sugai
Article

Summary

Cultures of unicellular algal flagellateEuglena gracilis grown in different conditions were subjected to action spectroscopy for step-down and step-up photophobic responses, respectively. The spectral region was extended into the UV-B/C as well as in the UV-A and visible regions with the Okazaki Large Spectrograph as the monochromatic light source. The photophobic responses of the cells were measured with an individual-cell assay method with the aid of a computerized video motion analyzer. In the UV-A and visible regions, the shapes of the action spectra were the so-called UV-A/blue type. In the newly studied UV-B/C region, new action peaks were found at 270 nm for the step-down response and at 280 nm for the step-up one. The absorption spectrum of flavin adenine dinucleotide (FAD) appeared to fit the action spectrum for the step-up response, whereas the shape of the step-down action spectrum, which has a UV-A peak (at 370 nm) higher than the blue peak (at 450 nm), appeared to be mimicked by the absorption spectrum of a mixed solution of 6-biopterin and FAD. These observations might also account for the fact that the UV-B/C peak wavelength at 270 nm of the action spectrum for the step-down response is shorter by 10 nm than the action spectrum for the step-up response at 280 nm.

Keywords

Action spectra Blue light Euglena Photophobic responses Ultraviolet light 

Abbreviations

FAD

flavin adenine dinucleotide

FWHM

spectral full width at half maximum

NIBB

National Institute for Basic Biology

OLS

Okazaki Large Spectrograph

PFB

paraflagellar body

UV-A

ultraviolet light of spectral region between 320 and 400 nm

UV-B/C

ultraviolet light of spectral region between 190 and 320 nm

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • S. Matsunaga
    • 1
  • T. Hori
    • 1
  • T. Takahashi
    • 2
  • M. Kubota
    • 3
  • M. Watanabe
    • 3
  • K. Okamoto
    • 4
  • K. Masuda
    • 4
  • M. Sugai
    • 4
  1. 1.Institute of Biological SciencesUniversity of TsukubaIbarakiJapan
  2. 2.Japan Advanced Institute of Science and TechnologyIshikawa
  3. 3.National Institute for Basic BiologyAichi
  4. 4.Department of Biology, Faculty of ScienceToyama UniversityToyama

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