, Volume 204, Issue 3–4, pp 128–138 | Cite as

Cytochemical localization of cellulase activity in pollen mother cells of david lily during meiotic prophase I and its relation to secondary formation of plasmodesmata

  • X. -Y. Wang
  • G. -Q. Guo
  • X. -W. Nie
  • G. -C. ZhengEmail author


Cellulase activity was localized at the ultrastructural level in pollen mother cells (PMCs) of David lily [Lilium davidii var.willmottiae (Wilson) Roffill] at different stages of meiotic prophase I. The enzyme was observed to appear at the early leptotene stage and reached its highest level at the subsequent zygotene stage, and its subcellular distribution revealed by the presence of electron-dense deposits of reaction product was found to be restricted exclusively to the endoplasmic reticulum (ER), the vesicles derived from that, and the cell wall, especially at the sites of secondary plasmodesmata and cytoplasmic channels where the wall was being digested. Other cytoplasmic organelles, such as dictyosomes and Golgi vesicles, lacked such deposits of reaction product. After zygotene the enzyme activity decreased abruptly, and at the pachytene stage only very few deposits could be observed in the cell wall. Our results indicate that cellulase is synthesized on rough ER and secreted directly via the smooth ER and ER-derived vesicles into the cell wall by exocytosis, where it brings about local wall breakdown, leading to the secondary formation of plasmodesmata and cytoplasmic channels.


Cellulase Cytochemical localization Cytoplasmic channel Lilium davidii var.willmottiae (Wilson) Roffill Pollen mother cell Secondary plasmodesma 


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  1. Bal AK (1972) Localization of cellulase in plant cells. In: Takeuchi T, Agawa K, Frigita S (eds) Proceedings of the 4th International Congress for Histochemistry and Cytochemistry, Kyoto, Japan, pp 301–302Google Scholar
  2. — (1974) Cellulase. In: Hayat MA (ed) Electron microscopy of enzymes, vol 3. Van Nostrand Reinhold, New York, pp 68–76Google Scholar
  3. —, Verma DPS, Byrne H, Maclachlan GA (1976) Subcellular localization of cellulase in auxin-treated pea. J Cell Biol 69: 97–105PubMedGoogle Scholar
  4. Binding H, Witt D, Monzer J, Mordhorst, Kollmann R (1987) Plant cell graft chimeras obtained by co-culture of isolated protoplasts. Protoplasma 141: 64–73Google Scholar
  5. Brummell DA, Catala C, Lashbrook CC, Bennett AB (1997) A membrane-anchored E-type endo-1,4-glucanase is localized on Golgi and plasma membranes of higher plants. Proc Natl Acad Sci USA 94: 4794–4799PubMedGoogle Scholar
  6. Carr DJ (1976) Plasmodesmata in growth and development. In: Gunning BES, Robards AW (eds) Intercellular communication in plants: studies on plasmodesmata. Springer, Berlin Heidelberg New York, pp 243–249Google Scholar
  7. Cheng KC (1956) On the process of intercellular migration of chromatin substance and new formation of nucleus in the pollen mother cells ofLillium sutchueneuse Franch. Sci Sin 5: 497–507Google Scholar
  8. —, Nie SW, Chen SW, Jian LC, Sun LH, Sun DL (1987) Studies on the secondary formation of plasmodesmata between the pollen mother cells of lily before cytomixis. Acta Biol Exp Sin 20: 1–11Google Scholar
  9. Ding B, Haudenshield JS, Hull RJ, Wolf S, Beachy RN, Lucas WJ (1992) Secondary plasmodesmata are specific sites of localization of the tobacco mosaic virus movement protein in transgenic tobacco plants. Plant Cell 4: 915–928PubMedGoogle Scholar
  10. — —, Willmitzer L, Lucas WJ (1993) Correlation between arrested secondary plasmodesmal development and onset of accelerated leaf senescence in yeast acid invertase transgenic tobacco plants. Plant J 4: 179–189PubMedGoogle Scholar
  11. Ehlers K, Kollmann R (1996) Formation of branched plasmodesmata in regeneratingSolatium nigrum protoplasts. Planta 199: 126–138Google Scholar
  12. Heslop-Harrison J (1966) Cytoplasmic continuities during spore formation in flowering plants. Endeavour 25: 65–72Google Scholar
  13. Jamieson JD, Palade G (1967) Intracellular transport of secretory protein in the pancreatic exocrine cells. J Cell Biol 34: 577PubMedGoogle Scholar
  14. Jones MGK (1976) The origin and development of plasmodesmata. In: Gunning BES, Robards AW (eds) Intercellular communication in plants: studies on plasmodesmata. Springer, Berlin Heidelberg New York, pp 81–105Google Scholar
  15. Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixation of high osmolality for use in electron microscopy. J Cell Biol 27: 137AGoogle Scholar
  16. Karp G (1996) Cell and molecular biology. Wiley, New YorkGoogle Scholar
  17. Kollmann R, Glockmann C (1985) Studies on graft unions I: plasmodesmata between cells of plants belonging to different unrelated taxa. Protoplasma 124: 224–235Google Scholar
  18. Kollmann R, Glockmann C (1991) Studies on graft unions III: on the mechanism of secondary formation of plasmodesmata at the graft interface. Protoplasma 165: 71–85Google Scholar
  19. —, Yang S, Glockmann C (1985) Studies on graft unions II: continuous and half plasmodesmata in different regions of the graft interface. Protoplasma 126: 19–29Google Scholar
  20. Lucas WJ, Ding B, van der School C (1993) Plasmodesmata and the supracellular nature of plants. New Phytol 125: 435–476Google Scholar
  21. Mezitt LA, Lucas WJ (1996) Plasmodesmal cell-to-cell transport of proteins and nucleic acids. Plant Mol Biol 32: 251–273PubMedGoogle Scholar
  22. Nessler CL, Mahlberg PG (1981) Cytochemical localization of cellulase activity in articulated, anastomosing laticifers ofPapaver somniferum L. (Papaveraceae). Am J Bot 68: 730–732Google Scholar
  23. Nie SW, Wang XY, Cheng KC (1984) Transmission and scanning electron microscopic observation on the plasmodesmal channels between the pollen mother cells of lily. Acta Bot Sin 26: 34–37Google Scholar
  24. Robards AW, Lucas WJ (1990) Plasmodesmata. Annu Rev Plant Physiol Plant Mol Biol 41: 369–419Google Scholar
  25. Wu, BJ, Xie, MT, Cui, YY, Chen, YP, Guo, WH, Jiang, H, Cheng, KC (1995) Formation of secondary plasmodesmata and intercellular passages by means of ACHT. Sci China Ser B 38: 187–194Google Scholar
  26. Zheng GC (KC Cheng), Yang CL, Zheng YR (1987) The relationship between cytomixis, chromosome mutation and karyotype evolution on lily. Caryologia 40: 243–259Google Scholar

Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • X. -Y. Wang
    • 1
  • G. -Q. Guo
    • 1
  • X. -W. Nie
    • 1
  • G. -C. Zheng
    • 1
    Email author
  1. 1.Cell Biology LaboratoryLanzhou UniversityLanzhouPeople's Republic of China

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