Protoplasma

, Volume 129, Issue 2–3, pp 188–197 | Cite as

Effects of osmotic conditions on ultrastructure of rat mammary epithelium

  • D. P. Dylewski
  • Th. W. Keenan
Article
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Accepted:

Summary

Effects of osmotic conditions on secretion of milk serum were examined using standard transmission electron microscopy. Rat mammary glands were infused with hyper-, iso-, and hypo-osmotic solutions. The intramammary infusion of these agents elicited distinct and repeatable morphological responses from lactating epithelial cells. The response to hyperosmolarity was an increase in compound exocytotic figures and an increase in secretory vesicle size (¯x=1.65 Μm in diameter). Glands infused with hypo-osmolar solutions exhibited the opposite response; reduction in compound exocytotic figures and reduced vesicle size (¯x=0.34 Μn in diameter). The response to iso-osmotic solutions was indistinguishable from untreated control tissue. The ratio of vesicular projections to depressions (vesicle membrane/plasma membrane interactions) could be experimentally altered through the intramammary infusion of solutions with different osmotic potentials. These observations support the suggestion that osmotic conditions may influence exocytosis of milk serum.

Keywords

Mammary epithelium Osmotic gradient Secretory vesicle 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • D. P. Dylewski
    • 1
  • Th. W. Keenan
    • 2
  1. 1.Department of Plant Pathology, Physiology and Weed ScienceVirginia TechUSA
  2. 2.Department of Biochemistry and NutritionVirginia TechBlacksburgUSA

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