Summary
Microplasmodia ofPhysarum polycephalum Schw differentiate into numerous hard walled spherules when deprived of nutrients. Cleavage occurs by invagination of the plasmalemma and growth of furrows centripetally. Cleavage furrows consistently contain ruthenium red positive fibrillar material. As the spherules become more rounded, a compact layer of ruthenium red positive material surrounds their membranes. A spherule wall which does not bind ruthenium red is deposited inside of the stained layer. Clusters of spherules are enclosed by ruthenium red positive material similar to the microplasmodial slime.
Keywords
Ruthenium Positive Material Compact Layer Fibrillar Material Spherule Wall
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