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Protoplasma

, Volume 212, Issue 1–2, pp 115–134 | Cite as

Arabinogalactan protein and wall-associated kinase in a plasmalemmal reticulum with specialized vertices

  • J. Scott Gens
  • Masaaki Fujiki
  • Barbara G. PickardEmail author
Original Papers

Summary

Arabinogalactan protein and wall-associated kinase (WAK) are suspected to be regulatory players at the interface between cytoplasm and cell wall. Both WAK(s) and arabinogalactan shown likely to represent arabinogalactan protein(s) have been visualized there with computational optical-sectioning microscopy. The arabinogalactan occurs in a polyhedral array at the external face of the cell membrane. WAK, and other proteins as yet unidentified, appear to fasten the membrane to the wall at vertices of the array. Evidence is presented that the array bears an important part of the mechanical stress experienced by the membrane, and it is speculated that the architectural organization of arabinogalactan protein, WAK, and other components of the array is critical for coordination of endomembrane activities, growth, and differentiation. The array has been named the plasmalemmal reticulum.

Keywords

Arabinogalactan protein BY-2 tobacco cells Computational optical-sectioning microscopy Plasmalemmal reticulum Wall-associated kinase 

Abbreviations

AGP

arabinogalactan protein

(β-D-Glc)3

β-D-glucosyl Yariv phenylglycoside

(β-D-Man)3

β-D-mannosyl Yariv phenylglycoside

SDS-AGE

sodium dodecyl sulfate-agarose gel electrophoresis

SDS-PAGE

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

WAK

wall-associated kinase

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Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • J. Scott Gens
    • 1
  • Masaaki Fujiki
    • 1
  • Barbara G. Pickard
    • 1
    Email author
  1. 1.The Gladys Levis Allen Laboratory of Plant Sensory Physiology, Biology DepartmentWashington UniversitySt. Louis

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