Protoplasma

, Volume 205, Issue 1–4, pp 101–106 | Cite as

Contribution of a plasma membrane redox system to the superoxide production by wheat root cells

  • F. V. Minibayeva
  • O. P. Kolesnikov
  • L. K. Gordon
Article

Summary

Wound stress activated wheat root cells to produce oxygen radicals. The production was accompanied by an increased permeability for potassium ions and a depolarization of the plasma membrane. Various electron donors, such as the nonpenetrating donor potassium ferrocyanide as well as NADH and NADPH, caused the amplification of superoxide production by root cells. The\(O_2 ^{. - } \)-generating system in wheat root cells was found to be considerably sensitive to diphenylene iodonium, which is generally considered as a suicide inhibitor of neutrophil NADPH oxidase, and to other inhibitors of flavoprotein activity, chlorpromazine and quinine. The xenobiotic compound amidopyrine caused activation of the\(O_2 ^{. - } \)-generating system, which was depressed by DPI. The\(O_2 ^{. - } \)-generating system in root cells was shown to be significantly dependent on calcium content. Calcium loading of the root cells induced a powerful increase of the superoxide release. Data obtained indicate that superoxide generation is one of the early events of the wound stress response. Redox systems of the plasma membrane may be involved in the superoxide production in response to wound stress and detoxification of xenobiotic compounds in root cells.

Keywords

Superoxide production Wheat roots Wound stress Plasma membrane redox activity 

Abbreviations

DPI

diphenylene iodonium

MP

membrane potential

\(O_2 ^{. - } \)

superoxide anion radical

ROS

reactive-oxygen species

SOD

superoxide dismutase

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • F. V. Minibayeva
    • 1
  • O. P. Kolesnikov
    • 1
  • L. K. Gordon
    • 1
  1. 1.Institute of Biochemistry and BiophysicsRussian Academy of SciencesKazanRussian Federation

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