Production of single-cell protein by the cultivation of yeast in anaerobic digester supernatant supplemented with carbohydrates
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Summary
A medium called basal medium (BM), which is suitable for the turbidimetric estimation of the growth of yeasts, was prepared from liquor obtained from the anaerobic digester of a waster treatment plant. BM was found to be rich in ammonia and contained a small amount of protein. The yeast,Canida utilis, grew well in this medium when a source of carbohydrate was added. Initial concentrations of 2.0% glucose (w/v) or 1.8% molasses (v/v) gave optimal growth. The yeast also utilized fructose and sucrose. Growth was accompanied by the removal of ammonia and a decrease in pH.
From the data obtained it may be calculated that 100 g of sucrose would yield 34.4 g of dried yeast cells or approximately 17.2 g of protein. One hundred grams of molasses would yield 40.5 g of dried yeast cells and approximately 20.3 of protein.
This process, in addition to producing protein, would also be of value in waste treatment in that it would ease the pollution of streams and rivers by ammonia and nitrate released from the waste treatment plants.
Keywords
Glucose Ammonia Nitrate Sucrose CarbohydratePreview
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