Effect of temperature shock on nuclear phenomena in microspores ofNicotiana tabacum and consequently on plantlet production
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In cytological investigations on the effect of cold-treatment in anther cultures ofNicotiana tabacum var. Badischer Burley, anthers were examined immediately after harvest, after 3 days of cold-incubation (5 °C) of the cultured anthers and after 4 more days of culture at 28 °C. These anthers were compared with untreated anthers at corresponding times. It was found that in those subjected to cold-treatment, developmental stages lagged behind comparable controls. A range of cytological types was seen in the seven day cultures. The differences in cytology and percentage viability were found to be directly related to differences in plantlet production between the two sets of cultures after incubation of a further 7 weeks. The delay in development and consequent increase in number of viable units is suggested as being the essential mechanism whereby haploid production is increased by cold-treatment.
KeywordsDevelopmental Stage Anther Culture Consequent Increase Comparable Control Temperature Shock
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