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Protoplasma

, Volume 136, Issue 2–3, pp 161–169 | Cite as

Phosphorylation-dephosphorylation is involved in Ca2+-controlled cytoplasmic streaming of characean cells

  • Y. Tominaga
  • R. Wayne
  • H. Y. L. Tung
  • M. Tazawa
Article

Summary

The mechanism of Ca2+ regulation of the cytoplasmic streaming in characean cells was studied in relation to protein phosphorylation and dephosphorylation. A tonoplast-free cell model was developed which was sensitive to Ca2+. Protein phosphatase-1 and its inhibitor-1 were applied into the tonoplast-free cells. A synthetic inhibitor of protein phosphatase, α-naphthylphosphate, was applied either to tonoplast-free cells from inside or to the outside of plasmalemma-permeabilized cells which are known to be very sensitive to Ca2+. ATP-γ-S applied to permeabilized cells strongly inhibited the recovery of the streaming which had been stopped by 10 μ M Ca2+. Both inhibitor-l and α-naphthylphosphate inhibited the streaming even in the absence of Ca2+. On the other hand, protein phosphatase-l recovered the streaming even in the presence of Ca2+.

The results indicate that characean streaming is regulated by the phosphorylation state of a regulatory and/or motile protein component. Streaming is activated when the component is dephosphorylated and inactivated when the component is phosphorylated. Ca2+ is assumed to stimulate both phosphorylation and dephosphorylation of the component. Involvement of Ca2+/calmodulin in the streaming recovery was discussed in terms of the stimulation of dephosphorylation.

Keywords

Characeae Ca2+ Cytoplasmic streaming Protein phosphatase Protein phosphatase inhibitor 

Abbreviations

ATP

γ-S, Adenosine-5′-O-(3-thiotriphosphate)

α-NP

α-naphthylphosphate

EGTA

ethylenglycol-bis-(β-aminoethylether)N,N′-tetraacetic acid

PIPES

piperazine-N,N′-bis(2-ethanesulfonic acid)

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Y. Tominaga
    • 1
    • 6
  • R. Wayne
    • 2
    • 3
  • H. Y. L. Tung
    • 4
    • 6
  • M. Tazawa
    • 5
    • 6
  1. 1.St. Agnes' Jr. College for WomenKyoto
  2. 2.The Department of BotanyThe University of TexasAustin
  3. 3.The Department of BotanyThe University of MassachusettsAmherst
  4. 4.Clayton Foundation Biochemical InstituteThe University of TexasAustin
  5. 5.Department of Botany, Faculty of ScienceUniversity of TokyoTokyo
  6. 6.Department of Biology, Faculty of ScienceThe University of TokyoTokyoJapan

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