, Volume 89, Issue 3–4, pp 339–351

The flagellar apparatus and striated rhizoplast of the zoospore ofOlpidium brassicae

  • Lene Lange
  • Lauritz W. Olson


The flagellar apparatus and its associated structures of the zoospore ofOlpidium brassicae are described and compared with observations of other zoospores of the uniflagellatePhycomycetes. The zoospore ofO. brassicae is shown to have an extensive cone-shaped rhizoplast fused to both the functional and the vestigial kinetosomes. Three-dimensional reconstructions were made of the kinetosomal region. The vestigial kinetosome differs from the functional, as it only has triplet bundles of microtubules and it lacks a system of props. The proximal termination of the central pair of flagellar microtubules occurs within the axoneme. No terminal plate is observed. The occurrence of dictyosomes in theChytridiales, Monoblepharidales, andHyphochytriales is discussed and it is concluded that a dictyosome may be present in the encysting zoospore and the maturing zoosporangium ofO. brassicae but only vestiges of a dictyosome are to be found in the free-swimming zoospore.


Flagellum rhizoplast rootlet zoospore microtubule Phycomycete 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bracker, C. E., 1967: Ultrastructure of fungi. Ann. Rev. Phytopathol.5, 342–374.Google Scholar
  2. Cantino, E. C., andL. C. Truesdell, 1971: Cytoplasmic gamma-like particles and other ultrastructural aspects of zoospores ofBlastocladiella britannica. Trans. brit. mycol. Soc.56, 169–179.Google Scholar
  3. Chambers, J. C., andL. C. Willoughby, 1964: The fine Structure ofRhizophlyctis rosea, a soil Phycomycete. J. roy. microscop. Soc.83, 355–364.Google Scholar
  4. Chong, J., andD. J. S. Barr, 1973: Zoospore development and fine structures inPhlyctochytrium arcticum (Chytridiales). Canad. J. Bot.51, 1411–1420.Google Scholar
  5. — —, 1974: Ultrastructure of the zoospores ofEntophlyctis confervae-glomeratae, Rhizophydium patellarium, andCatenaria anguillulae. Canad. J. Bot.52, 1197–1204.Google Scholar
  6. Fuller, M. S., 1966: Structure of the uniflagellate zoospores of aquaticPhycomycetes. Proc. Symp. Colston Res. Soc.18, 67–84.Google Scholar
  7. —, andR. Reichle, 1965: The zoospore and early development ofRhizidiomyces apophysatus. Mycologia57, 946–961.Google Scholar
  8. — —, 1968: The fine structure ofMonoblepharella sp. zoospores. Canad. J. Bot.46, 279–283.Google Scholar
  9. —, andL. W. Olson, 1971: The zoospore ofAllomyces. J. gen. Microbiol.66, 171–183.Google Scholar
  10. Gibbons, I. R., andA. V. Grimstone, 1960: On flagellar structure in certain flagellates. J. biophys. biochem. Cytol.11, 195–205.Google Scholar
  11. Held, A. A., 1975: The zoospore ofRozella allomycis: ultrastructure. Canad. J. Bot.53, 2212–2232.Google Scholar
  12. Kazama, F. Y., 1972: Ultrastructure and phototaxis of the zoospores ofPhlyctochytrium sp., an estuarine chytrid. J. gen. Microbiol.71, 555–566.Google Scholar
  13. Koch, W. J., 1956: Studies of the motile cells of chytrids. I. Electron microscope observations of the flagellum, blepharoplast, and rhizoplast. Amer. J. Bot.43, 811–819.Google Scholar
  14. Lesemann, D. E., andW. H. Fuchs, 1970: Elektronenmikroskopische Untersuchung über die Vorbereitung der Infektion in encystierten Zoosporen vonOlpidium brassicae. Arch. Mikrobiol.71, 9–19.Google Scholar
  15. Lessie, P. E., andJ. S. Lovett, 1968: Ultrastructural changes during sporangium formation and zoospore differentiation inBlastocladiella emersonii. Amer. J. Bot.55, 220–236.Google Scholar
  16. Manton, I., B. Clarke, A. D. Greenwood, andE. A. Flint, 1952: Further observations on the structure of plant cilia by a combination of visual and electron microscopy. J. exp. Bot.3, 204–215.Google Scholar
  17. Moore, R. T., 1965: Distribution and characterization of the Golgi complex in thePhycomycetes. J. Cell Biol.27, 69 A (Abstr.).Google Scholar
  18. Olson, L. W., 1973: The meiospore ofAllomyces. Protoplasma78, 113–127.PubMedGoogle Scholar
  19. —, andM. S. Fuller, 1968: Ultrastructural evidence for the biflagellate origin of the uniflagellate fungal zoospore. Arch. Mikrobiol.62, 237–250.PubMedGoogle Scholar
  20. Pitelka, D. R., and F. M.Child, 1964: Locomotor apparatus of ciliates and flagellates. In: Biochemistry and Physiology of Protozoa (S. H.Hutner, ed.). N.Y. Acad. Press3, 131–198.Google Scholar
  21. Reichle, R. E., 1972: Fine structure ofOedogoniomyces zoospores, with comparative observations onMonoblepharella zoospores. Canad. J. Bot.50, 819–824.Google Scholar
  22. —, andM. S. Fuller, 1967: The fine structure ofBlastocladiella emersonii zoospores. Amer. J. Bot.54, 81–92.Google Scholar
  23. Temmink, J. H. M., andR. N. Campbell, 1969: The ultrastructure ofOlpidium brassicae. II. Zoospores. Canad. J. Bot.47, 227–231.Google Scholar
  24. Umphlett, C. J., andL. W. Olson, 1967: Cytological and morphological studies of new species ofPhlyctochytrium. Mycologia59, 1085–1096.Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Lene Lange
    • 1
    • 2
  • Lauritz W. Olson
    • 1
    • 2
  1. 1.Institute for SporeplanterUniversity of CopenhagenCopenhagen KDenmark
  2. 2.Genetic InstituteUniversity of CopenhagenCopenhagen KDenmark

Personalised recommendations