Plant Cell Reports

, Volume 16, Issue 9, pp 624–627 | Cite as

RAPD analysis of somaclonal variants derived from embryo callus cultures of peach

  • G. Hashmi
  • R. Huettel
  • R. Meyer
  • L. Krusberg
  • F. Hammerschlag
Article

Abstract

Peach [Prunus persica (L.) Batsch] regenerants from cv ‘Sunhigh’ embryo no. 156, regenerants obtained from cv ‘Redhaven’ embryo no. 30, and two peach cultivars ‘Sunhigh’ and ‘Redhaven’, were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with up to 60 10-mer primers. Although 35 primers produced results with scoreable bands, only 10 of the primers revealed polymorphism for regenerants of embryo no. 156 and cv ‘Sunhigh’, and 1 revealed a low level of polymorphism for regenerants of embryo no. 30 and cv ‘Redhaven’. This study demonstrates the feasibility of using RAPD markers to identify somaclonal variants of peach and provides evidence for the existence of genetic differences among these variants.

Key words

Prunus persica Somaclonal variation 

Abbreviations

PCR

Polymerase chain reaction

RAPD

random amplified polymorphic DNA

RFLP

Restriction fragment length polymorphism

CTAB

cetyltrimethyl ammonium bromide

PVP

polyvinyl pyrolidone

dNTP

deoxy-ribonucleotide triphosphate

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • G. Hashmi
    • 1
    • 2
  • R. Huettel
    • 3
  • R. Meyer
    • 3
  • L. Krusberg
    • 4
  • F. Hammerschlag
    • 5
  1. 1.Plant Molecular Biology and Nematology Laboratories USDA/ARSBeltsvilleUSA
  2. 2.Department of BotanyUniversity of MarylandCollege ParkUSA
  3. 3.Nematology Laboratories, USDA/ARSBeltsvilleUSA
  4. 4.Department of BotanyUniversity of MarylandCollege ParkUSA
  5. 5.Plant Molecular Biology, USDA/ARSBeltsvilleUSA

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