Radiation and Environmental Biophysics

, Volume 34, Issue 2, pp 121–125

No indications of an enhanced UV-light-induced unscheduled DNA synthesis in splenocytes of mice following a low-dose irradiation in vivo or in vitro

  • A. Wojcik
  • C. A. Seemayer
  • W. -U. Müller
  • C. Streffer
Original Paper

Abstract

One of the open questions regarding the adaptive response to ionizing radiation is whether it can be induced in G0 lymphocytes. In the majority of experiments in which an adaptive response in G0 lymphocytes was observed, the adapting dose was applied in vivo. In order to investigate whether there is some in vivo component of adaptive response, mouse splenocytes of the C57BL/6 strain were irradiated with 0.1 Gy x-rays either in vivo or in vitro, and their UV-light-induced unscheduled DNA synthesis (UDS) levels were determined autoradiographically. An augmented UV-light-induced UDS following an adapting dose applied in vivo has previously been described by several authors in splenocytes of C57BL/6 mice, indicating that the adapting dose enhanced the DNA repair capacity of lymphocytes. In the present investigation, however, no evidence of an adaptive response could be seen regardless of whether the adapting dose was given in vivo or in vitro. Those results present a further indication for the fact that the adaptive response to ionizing radiation is not always inducible, even in lymphocytes of an inbred mouse strain in which its existence has been reported before.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • A. Wojcik
    • 1
  • C. A. Seemayer
    • 1
  • W. -U. Müller
    • 1
  • C. Streffer
    • 1
  1. 1.Klinikum EssenInstitut für Medizinische StrahlenbiologieEssenGermany

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