, Volume 127, Issue 1–2, pp 128–132 | Cite as

Structure and function in the grass ligule: The membranous ligule ofLolium temulentum L. as a secretory organ

  • N. Chaffey


Using phosphotungstic acid and periodic acid/thiocarbohydrazide/silver proteinate (Thiéry test) aspects of polysaccharide ultracytochemistry were studied in the membranous ligule ofLolium temulentum L. Staining results are presented for all three tissues-abaxial and adaxial epidermes and mesophyll-but discussed only for the epidermes. PTA- and PATAg-staining of the adaxial epidermis suggested synthesis of a conjugated polysaccharide material in this tissue, its accumulation in the periplasmic space and its subsequent secretion to the outside of the ligule via gaps in the cuticle. The ligule of this grass is considered to be a secretory organ.


Ligule Lolium temulentum L. Poaceae Polysaccharide cytochemistry Secretion Ultrastructure 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • N. Chaffey
    • 1
  1. 1.Department of BotanyUniversity of Durham, Science SiteDurhamUK

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