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Protoplasma

, Volume 127, Issue 1–2, pp 93–100 | Cite as

Mechanism of inhibition of cytoplasmic streaming by myrmicacin (Β-hydroxydecanoic acid) inChara andSpirogyra

  • Teruo Shimmen
  • Masashi Tazawa
Article

Summary

Myrmicacin (Β-hydroxydecanoic acid) applied externally inhibited the cytoplasmic streaming ofChara cells in acidic (pH 4.5) but not in neutral (pH 7.5) solution. Application by intracellular perfusion at neutral pH also did not inhibit the streaming. Other carboxylic acids could inhibit the streaming when given as an acidic external solution. Inhibition of the cytoplasmic streaming was concluded to occur due to acidification of the intracellular pH upon application of an acidic external medium of these carboxylic acids. The cytoplasmic streaming ofSpirogyra was also inhibited by myrmicacin and acetic acid in acidic solution.

Keywords

Chara Cytochalasin B Cytoplasmic pH Cytoplasmic streaming Myrmicacin Spirogyra 

Abbreviations

APW

artificial pond water

CB

cytochalasin B

DMGA

3.3-dimethylglutaric acid

DMSO

dimethylsulfoxide

EGTA

ethyleneglycol-bis-(\-aminoethyl ether)N

N′

tetraacetic acid

HEPES

2-hydroxyethylpiperazine

N′

ethanesulfonic acid

MES

2-(N-morpholino)ethanesulfonic acid

pHi

intracellular pH

Tris

trishydroxyaminomethane

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Teruo Shimmen
    • 1
  • Masashi Tazawa
    • 1
  1. 1.Department of Botany, Faculty of ScienceUniversity of TokyoTokyoJapan

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