Advertisement

Molecular and Cellular Biochemistry

, Volume 116, Issue 1–2, pp 53–58 | Cite as

Phosphatidylcholine metabolism in ischemic and hypoxic hearts

  • Patrick C. Choy
  • Monroe Chan
  • Grant Hatch
  • Ricky Y. K. Man
Article

Abstract

The rates of phosphatidylcholine biosynthesis in the isolated hamster hearts under ischemic and hypoxic conditions were examined. Global ischemia was produced by perfusion of the heart with a reduced flow, whereas hypoxia was produced by perfusion with a N2-saturated buffer. A 51% reduction in the biosynthesis of phosphatidylcholine was observed in the ischemic heart. The reduction was caused by a severe decrease in ATP level which resulted in a diminished conversion of choline into phosphocholine. A 22% reduction in the biosynthetic rate of phosphatidylcholine was also detected in the hypoxic heart. The reduction was caused by a diminished level of CTP which resulted in a decreased conversion of phosphocholine to CDP-choline. No compensatory mechanism was triggered during ischemia, but the CTP: phosphocholine cytidylyltransferase activity was enhanced in the hypoxic heart. Our results demonstrate the possible rate-limiting role of choline kinase and reconfirm the regulatory role of the cytidylyltransferase in the biosynthesis of phosphatidylcholine. (Mol Cell Biochem116: 53–58, 1992)

Key words

phosphatidylcholine biosynthesis ischemia hypoxia hamster heart 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    White DA: The phospholipid composition of mammalian tissues. In: Ansell GB, Hawthorne JN, Dawson RMC (eds) Form and function of phospholipids. Elsevier Scientific Publishing, Amsterdam, 1973, pp 441–483Google Scholar
  2. 2.
    Cullis PR, Hope MJ: Physical properties and functional roles of lipids in membranes. In: Vance DE, Vance JE (eds) Biochemistry of lipids and membranes. Benjamin Cummings Publishing, Menlo Park, CA, 1985, pp 25–72Google Scholar
  3. 3.
    Coleman R: Membrane-bound enzymes and membrane ultrastructure. Biochem Biophys Acta 300: 1–30, 1973Google Scholar
  4. 4.
    Berridge MJ, Irvine RF: Inositol triophosphate, a novel second messenger in cellular signal transduction. Nature (London) 312: 315–321, 1984Google Scholar
  5. 5.
    Exton JH: Signalling through phosphatidylcholine breakdown. J Biol Chem 265: 1–4, 1990Google Scholar
  6. 6.
    Dhalla NS, Ziegelhoffer A, Harrow JAC: Regulatory role of membrane systems in heart function. Can J Physiol Pharm 55: 1211–1234, 1977Google Scholar
  7. 7.
    Vance DE: Phospholipid metabolism in eucaryotes. In: Vance DE, Vance JE (eds) Biochemistry of lipids and membranes. Benjamin Cummings Publishing, Menlo Park, CA, 1985, pp 25–72Google Scholar
  8. 8.
    Zelinski TA, Savard JD, Man RYK, Choy PC: Phosphatidylcholine biosynthesis in isolated hamster hearts. J Biol Chem 255: 11423–11428, 1980Google Scholar
  9. 9.
    Vance DE, Choy PC: How is phosphatidylcholine biosynthesis regulated? Trends Biochem Sci 4: 145–148, 1979Google Scholar
  10. 10.
    Hatch GM, O K-M, Choy PC: Regulation of phosphatidylcholine metabolism in mammalian hearts. Biochem Cell Biol 67: 67–77, 1989Google Scholar
  11. 11.
    Paddon HB, Vance DE: The relationship between cholinephosphate phosphatase alkaline phosphatase and phosphatidylcholine biosynthesis in HeLa cells. Biochim Biophys Acta 488: 181–189, 1977Google Scholar
  12. 12.
    Arthur G, Choy PC: Acyl specificity of hamster heart CDPcholine: 1,2-diacylglycerol phosphocholine transferase in phosphatidylcholine biosynthesis. Biochim Biophys Acta 795: 221–229, 1984Google Scholar
  13. 13.
    Hatch GM, Choy PC: Effect of hypoxia on phosphatidylcholine biolsynthesis in the isolated hamster heart. Biochem J 268: 47–54, 1990Google Scholar
  14. 14.
    Piper HM, Schwartz P, Hutter J, Spieckermann PG: Energy metabolism and enzyme release of cultured adult rat heart muscle cells during anoxia. J Mol Cell Cardiol 16: 995–1007, 1984Google Scholar
  15. 15.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951Google Scholar
  16. 16.
    Brophy PJ, Choy PC, Toone JR, Vance DE: Choline kinase and ethanolamine kinase are separate soluble enzymes in rat liver. Eur J Biochem 78: 491–495, 1977Google Scholar
  17. 17.
    Vance DE, Pelech SD, Choy PC: CTP: phosphocholine cytidylyltransferase from rat liver. Methods Enzymol 71: 576–581, 1981Google Scholar
  18. 18.
    O K-M, Choy PC: Solubilization and partial purification of cholinephosphotransferase in hamster tissues. Lipids 25: 122–124, 1990Google Scholar
  19. 19.
    Choy PC: Control of phosphatidylcholine biosynthesis in myopathic hamster hearts. J Biol Chem 257: 10928–10933, 1982Google Scholar
  20. 20.
    Choy PC, Paddon HB, Vance DE: An increase in cytoplasmic CTP accelerates the reaction catalyzed by CTP: phosphocholine cytidylyltransferase in poliovirus-infected HeLa cells. J Biol Chem 255: 1070–1073, 1980Google Scholar
  21. 21.
    Vance DE: CTP: cholinephopshate cytidylyltransferase. In: Vance DE (ed) Phosphatidylcholine metabolism, CRC Press, Boca Raton, FL. 1989, pp 33–46Google Scholar
  22. 22.
    Slight R, Kent CR: Regulation of phosphatidylcholine biolsynthesis in mammalian cells. I. Effects of phospholipase C treatment on phosphatidylcholine metabolism in chinese hamster ovary cells and LM mouse fibroblasts. J Biol Chem 258: 824–830, 1983Google Scholar
  23. 23.
    Choy PC, Vance DE: Lipid requirements for activation of CTP phosphocholine cytidylyltransferase from rat liver. J Biol Chem 253: 5163–5167, 1978Google Scholar
  24. 24.
    Vance DE, Pelech SL: Enzyme translocation in the regulation of phosphatidylcholine biosynthesis. Trends Biochem Sci 9: 17–20, 1984Google Scholar
  25. 25.
    Katz AM, Messineo FC: Lipid-membrane interactions and the pathogenesis of ischemic damage in the myocardium. Circ Res 48: 1–16, 1981Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Patrick C. Choy
    • 1
  • Monroe Chan
    • 1
  • Grant Hatch
    • 1
  • Ricky Y. K. Man
    • 1
  1. 1.Departments of Biochemistry & Molecular Biology and Pharmacology, Faculty of MedicineUniversity of ManitobaWinnipegCanada

Personalised recommendations