Molecular and Cellular Biochemistry

, Volume 116, Issue 1–2, pp 53–58 | Cite as

Phosphatidylcholine metabolism in ischemic and hypoxic hearts

  • Patrick C. Choy
  • Monroe Chan
  • Grant Hatch
  • Ricky Y. K. Man


The rates of phosphatidylcholine biosynthesis in the isolated hamster hearts under ischemic and hypoxic conditions were examined. Global ischemia was produced by perfusion of the heart with a reduced flow, whereas hypoxia was produced by perfusion with a N2-saturated buffer. A 51% reduction in the biosynthesis of phosphatidylcholine was observed in the ischemic heart. The reduction was caused by a severe decrease in ATP level which resulted in a diminished conversion of choline into phosphocholine. A 22% reduction in the biosynthetic rate of phosphatidylcholine was also detected in the hypoxic heart. The reduction was caused by a diminished level of CTP which resulted in a decreased conversion of phosphocholine to CDP-choline. No compensatory mechanism was triggered during ischemia, but the CTP: phosphocholine cytidylyltransferase activity was enhanced in the hypoxic heart. Our results demonstrate the possible rate-limiting role of choline kinase and reconfirm the regulatory role of the cytidylyltransferase in the biosynthesis of phosphatidylcholine. (Mol Cell Biochem116: 53–58, 1992)

Key words

phosphatidylcholine biosynthesis ischemia hypoxia hamster heart 


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Patrick C. Choy
    • 1
  • Monroe Chan
    • 1
  • Grant Hatch
    • 1
  • Ricky Y. K. Man
    • 1
  1. 1.Departments of Biochemistry & Molecular Biology and Pharmacology, Faculty of MedicineUniversity of ManitobaWinnipegCanada

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