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Journal of Neurocytology

, Volume 10, Issue 4, pp 589–605 | Cite as

Serial analysis of microtubules in cultured rat sensory axons

  • Drnnis Bray
  • Mary Bartlett Bunge
Article

Summary

A fascicle of axons in an organotypic culture of rat dorsal root ganglion was analysed in 190 serial transverse sections, a total fascicle length calculated to be 12.8μm. The number, position and continuity of microtubules and components of smooth endoplasmic reticulum (SER) were examined. The 187 microtubules traced in 27 axons showed a total of 32 terminations in the series; 14 of these were ‘starts’ and 18 were ‘stops’ in relation to progression away from the neuronal soma; three tubules began and ended in the series. Microtubules overall were long, their lengths calculated to average 108μm. Terminations usually occurred close to the plasma membrane but otherwise exhibited no obvious morphological specialization. Microtubules in some axons were concentrated in the periphery of the axon, a region from which neurofilaments were excluded. The relative position of tubules in the axon did not change in any systematic way (for example, by rotation) throughout the series. The SER showed marked variation in form, from the expected membrane-bounded vesicle to profiles that resembled a microtubule or a thinner densely stained filament.

Keywords

Plasma Membrane Endoplasmic Reticulum Relative Position Dorsal Root Ganglion Transverse Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman and Hall Ltd. 1981

Authors and Affiliations

  • Drnnis Bray
    • 1
  • Mary Bartlett Bunge
    • 1
  1. 1.Department of Anatomy and NeurobiologyWashington University School of MedicineSt LouisUSA

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