Planta

, Volume 201, Issue 1, pp 53–63 | Cite as

A non-invasive measurement of phloem and xylem water flow in castor bean seedlings by nuclear magnetic resonance microimaging

  • W. Köckenberger
  • J. M. Pope
  • Y. Xia
  • K. R. Jeffrey
  • E. Komor
  • P. T. Callaghan
Article

Abstract

A flow-sensitive nuclear magnetic resonance (NMR) microimaging technique was applied to measure directly the in-vivo water flow in 6-d-old castor bean seedlings. The achieved in-plane resolution of the technique allowed discrimination between xylem and phloem water flow. Both the xylem- and the phloem-average flow velocities in the intact seedling could be quantified. Furthermore, the total conductive cross-sectional area of the xylem vessels and the phloem sieve elements could be determined using the non-invasive and non-destructive NMR microimaging technique. Hence, it was possible to calculate the in-vivo volume flow rates for both xylem and phloem water flow. Our non-destructive technique showed that previously used methods to measure phloem water flow affected the flow rate itself. In the intact seedlings we found values of 16.6 μl·h−1, two fold lower than those previously estimated from phloem exudation rates. Finally, our results demonstrate for the first time that water is internally circulated between phloem and xylem, and that water flow within the xylem is maintained by this internally circulated water, even in the absence of any significant transpiration or evaporation.

Key words

Non-invasive measurement (water flow) Nuclear magnetic resonance microimaging Phloem water flow Ricinus seedling Xylem water flow 

Abbreviation

NMR

nuclear magnetic resonance

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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • W. Köckenberger
    • 1
  • J. M. Pope
    • 2
  • Y. Xia
    • 3
  • K. R. Jeffrey
    • 4
  • E. Komor
    • 1
  • P. T. Callaghan
    • 5
  1. 1.PflanzenphysiologieUniversität BayreuthBayreuthGermany
  2. 2.School of PhysicsQueensland University of TechnologyBrisbaneAustralia
  3. 3.Physics DepartmentOakland UniversityRochesterUSA
  4. 4.Department of PhysicsUniversity of GuelphGuelphCanada
  5. 5.Department of PhysicsMassey UniversityPalmerston NorthNew Zealand

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