Immunochemical studies of foot-and-mouth disease
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Polyethylene glycol (Carbowax 20M) was found to be an efficient precipitant of foot-and-mouth disease virus. Infected tissue culture fluids from baby hamster kidney-21 cells were clarified and polyethylene glycol added to 6% (w/v) concentration. The precipitate was collected, resuspended to 1/10 the original volume in buffer solution, and reprecipitated with 6% polyethylene glycol. The second precipitate was resuspended to 1/60 the original volume and the virus purified from these concentrates by two cycles of cesium chloride density gradient ultracentrifugation. The purified virus was physically characterized by analytical ultracentrifugation and spectroscopy. Immunological reactivity was established by immunodiffusion, complement fixation and immunogenicity. The procedure was used with several virus types. Yields of 12–65% of the original viral material were obtained and varied with the virus type.
KeywordsPolyethylene Glycol Virus Preparation Baby Hamster Kidney Cell Immunochemical Study Insoluble Debris
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