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Archiv für die gesamte Virusforschung

, Volume 30, Issue 4, pp 343–352 | Cite as

Immunochemical studies of foot-and-mouth disease

VII. Characterization of foot-and-mouth disease virus concentrated by polyethylene glycol precipitation
  • G. G. Wagner
  • J. L. Card
  • K. M. Cowan
Article

Summary

Polyethylene glycol (Carbowax 20M) was found to be an efficient precipitant of foot-and-mouth disease virus. Infected tissue culture fluids from baby hamster kidney-21 cells were clarified and polyethylene glycol added to 6% (w/v) concentration. The precipitate was collected, resuspended to 1/10 the original volume in buffer solution, and reprecipitated with 6% polyethylene glycol. The second precipitate was resuspended to 1/60 the original volume and the virus purified from these concentrates by two cycles of cesium chloride density gradient ultracentrifugation. The purified virus was physically characterized by analytical ultracentrifugation and spectroscopy. Immunological reactivity was established by immunodiffusion, complement fixation and immunogenicity. The procedure was used with several virus types. Yields of 12–65% of the original viral material were obtained and varied with the virus type.

Keywords

Polyethylene Glycol Virus Preparation Baby Hamster Kidney Cell Immunochemical Study Insoluble Debris 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Albertsson, P. A.: “Partition of Cell Particles and Macromolecules.” Wiley, New York, 1960.Google Scholar
  2. 2.
    Bachrach, H. L., andS. S. Breese, Jr.: In: “Methods in Virology”,K. Maramorosch andH. Koprowski, eds., vol. 4, p. 351, New York: Academic Press, 1968.Google Scholar
  3. 3.
    Bachrach, H. L., R. Trautman, andS. S. Breese, Jr.: Chemical and physical properties of virtually pure foot-and-mouth disease virus. Amer. J. vet. Res.25, 333–342 (1964).PubMedGoogle Scholar
  4. 4.
    Clark, M. F.: Purification and fractionation of alfalfa mosaic virus with polyethylene glycol. J. gen. Virol.3, 427–432 (1968).CrossRefPubMedGoogle Scholar
  5. 5.
    Cooper, P.: In: “Methods in Virology”,K. Maramorosch andH. Koprowski, eds., vol. 3, p. 244, New York: Academic Press, 1967.Google Scholar
  6. 6.
    Cowan, K. M.: Effect of Merthiolate on agar gel diffusion precipitin reactions with foot-and-mouth disease virus. J. Immunol.97, 647–653 (1966).PubMedGoogle Scholar
  7. 7.
    Cowan, K. M.: Immunochemical studies of foot-and-mouth disease. IV. Preparation and evaluation of antisera specific for virus, virus protein subunit and the infection-associated antigen. J. Immunol.101, 1183–1191 (1968).PubMedGoogle Scholar
  8. 8.
    Cowan, K. M., andR. Trautman: Immunochemical studies of foot-and-mouth disease. I. Complement-fixation reactions with isolated antigenic components. J. Immunol.99, 729–736 (1967).PubMedGoogle Scholar
  9. 9.
    Graves, J. H., K. M. Cowan, andR. Tbautman: Immunochemical studies of foot-and-mouth disease. II. Characterization of RNA-free virus-like particles. Virology34, 269–274 (1968).CrossRefPubMedGoogle Scholar
  10. 10.
    Hebert, T. T.: Precipitation of plant viruses by polyethylene glycol. Phytopathology53, 362 (1963).Google Scholar
  11. 11.
    Hyslop, N. St. G., andA. W. Morrow: The influence of aluminum hydroxide content, dose volume and the inclusion of saponin on the efficacy of inactivated foot-and-mouth disease vaccines. Res. Vet. Sci.10, 109–120 (1969).PubMedGoogle Scholar
  12. 12.
    Leberman, R.: The isolation of plant viruses by means of “simple” coacervates. Virology30, 341–347 (1966).CrossRefPubMedGoogle Scholar
  13. 13.
    MacPherson, I., andM. Stoker: Polyoma transformation of hamster cell clones- an investigation of genetic factors affecting cell competence. Virology16, 147–151 (1962).CrossRefPubMedGoogle Scholar
  14. 14.
    Polatnick, J., andH. L. Bachrach: Production and purification of milligram amounts of foot-and-mouth disease virus from baby hamster kidney cell cultures. Appl. Microbiol.4, 368–373 (1964).Google Scholar
  15. 15.
    VanKammen, A.: Purification and properties of the components of cowpea mosaic virus. Virology81, 633–642 (1967).CrossRefGoogle Scholar
  16. 16.
    Yamamoto, K. R., B. M. Alberts, R. Benzinger, L. Lawhorne, andG. Treiber: Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology40, 734–744 (1970).CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • G. G. Wagner
    • 1
  • J. L. Card
    • 1
  • K. M. Cowan
    • 1
  1. 1.Plum Island Animal Disease Laboratory, Animal Disease and Parasite Research Division, Agricultural Research ServiceU.S. Department of AgricultureGreenportUSA

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