Archiv für die gesamte Virusforschung

, Volume 42, Issue 1, pp 67–77 | Cite as

A small intranuclear virus recovered from lymphatic tissue of a bovine with malignant lymphoma

  • A. L. Burroughs
  • G. H. CardinetJr.
Article
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Summary

Madin-Darby Bovine Kidney (MDBK) cells were permanently infected with a virus following inoculation and long-term incubation with lymphatic tissue from a 7-year-old bovine with lymphocytic type of malignant lymphoma. The virus, passed to a calf by dripping a suspension into its eyes, nostrils, and mouth, was recovered from lymphatic tissue 106 days later. One of 6 calves inoculated with a quantity of concentrated virus suspension developed persistent lymphocytosis in approximately 2 years. Permanently infected MDBK cells of a line infected for several months were agglutinated by both concanavalin A and wheat germ lipase contaminant. Electron micrographs of thin sections of cells revealed many virions in the nuclei. Enveloped particles ranged from 47 to 58 mμ in diameter. Autoradiographic studies resulted in labelling the intranuclear virions of infected cells with uridine-5-3H. Incorporation of isotope was not inhibited by FUdR and actinomycin D.

Keywords

Lipase Infected Cell Lymphatic Tissue Malignant Lymphoma Concanavalin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • A. L. Burroughs
    • 1
  • G. H. CardinetJr.
    • 1
  1. 1.Department of Infectious Diseases and Physiological SciencesKansas State UniversityManhattanUSA

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