Archiv für die gesamte Virusforschung

, Volume 43, Issue 3, pp 191–199 | Cite as

The effect of certain amino acids on reproduction of encephalomyocarditis virus

II. Investigation of the inhibiting effect of L-histidine
  • Svetlana V. Maslova
  • Olga A. Medvedkina
  • G. A. Shirman
Article
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Summary

L-histidine in 2–4mm concentration inhibits reproduction of encephalomyocarditis (EMC) virus in suspension of Krebs-2 mouse-ascites-tumour cells at an incubation temperature of 41.0°–41.5° C but is not effective in these concentrations at a lower temperature (34.5° C). L-histidine prevents multiplication of EMC virus when added to the incubation medium at any period of the reproduction cycle. When the incubation temperature is reduced from 41.5° to 34.5°, virus reproduction may be restored after a certain lag period. L-histidine appears to inhibit synthesis of all classes of virus-specific RNA. The synthesis of cellular RNA in infected cells is inhibited in the presence of 1-histidine as compared with its level in uninfected cells. L-histidine does not inhibit markedly protein synthesis either in infected or in uninfected cells. Accumulation of labelled histidine in the acid-soluble fraction of the cells does not depend upon the incubation temperature to any significant degree, and the intracellular content of the amino acid does not change when the incubation temperature is reduced.

Keywords

Infectious Disease Protein Synthesis Histidine Infected Cell Virus Reproduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Svetlana V. Maslova
    • 1
  • Olga A. Medvedkina
    • 1
  • G. A. Shirman
    • 1
  1. 1.The U.S.S.R. Acad. Med. Sci. Institute of Poliomyelitis and Virus EncephalitidesMoscowUSSR

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