Archiv für die gesamte Virusforschung

, Volume 36, Issue 1–2, pp 166–176 | Cite as

Studies with a rhinovirus of bovine origin

I. growthin vitro of strain RS 3x
  • P. R. Ide
  • J. H. Darbyshire
Article
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Summary

Studies were made on the growthin vitro of a strain (RS 3x) of a bovine rhinovirus using several cell systems. The cytopathic effect in calf kidney (CK) cells occurred more rapidly when a medium containing low levels of sodium bicarbonate was employed, and this was more progressive in cultures incubated while being rolled than in those incubated in a stationary position. Infectivity titres attained by the virus in CK cells were initially about equal at both 33°C and 37°C, but higher and more persistent titres were subsequently obtained at 33° C. Cultures grown in the presence of bovine serum containing a neutralizing titre of 1:2000 or greater to the RS 3x strain were markedly inhibitory to virus growth, even when the cells were washed prior to inoculation and subsequently maintained in a serum-free medium. It was found necessary to change the culture medium to one which contained foetal calf serum, free of specific antibody, 3 days prior to infection with virus and to wash the cells at least 5 times before use. The RS 3x strain replicated in monolayer cultures of bovine origin as well as in the differentiated cells of calf nasal turbinate and tracheal organ cultures.

Keywords

Bicarbonate Foetal Calf Serum Calf Serum Organ Culture Specific Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • P. R. Ide
    • 1
  • J. H. Darbyshire
    • 2
  1. 1.Animal Pathology DivisionHealth of Animals BranchCanada
  2. 2.The Animal Virus Research InstitutePirbright, WokingEngland

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