Advertisement

Archiv für die gesamte Virusforschung

, Volume 36, Issue 3–4, pp 351–362 | Cite as

Parvoviruses as contaminants of permanent human cell lines

IV. Multiplication of KBSH-virus in KB-cells
  • G. Siegl
  • C. Hallauer
  • A. Novak
Article

Summary

The multiplication of KBSH-virus in KB cells has been investigated by means of immunofmorescence, histological, and ultrahistological techniques.

According to the results of immunofluorescence studies virus synthesis starts within the cytoplasm of an infected cell after a lag period of 5–6 hours. At that time viral antigens could be detected in the perinuclear region. During the following 6 to 8 hours increasing amounts of demonstrable antigens appeared within the nucleus and, due to the formation of brightly staining intranuclear inclusions paralleled by the reappearance of fluorescing granules in the cytoplasm, it had to be concluded that virus synthesis was completed within the nucleus after 14–20 hours following infection.

The formation of intranuclear inclusions could be readily demonstrated by means of H & E staining. At the ultrahistological level, affected nuclei showed severe signs of structural degeneration and were frequently filled with large arrays of both empty and full virus particles.

Regarding the relationship between the mitotic index of the cell monolayer at the time infection occurred and the outcome of virus replication, there was strong evidence that synthesis of KBSH-virus is favoured by cells undergoing active growth. In cells of limited physiologic activity virus replication was found to extend for more than 24 hours.

Keywords

Virus Replication Virus Particle Cell Monolayer Mitotic Index Human Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Al-Lami, F., N. Ledinko, andH. Toolan: Electron microscope study of human NB and SMH cells infected with the parvovirus H-1: Involvement of the nucleolus. J. gen. Virol.5, 485–492 (1969).Google Scholar
  2. 2.
    Bernhard, W., F. H. Kasten etCh. Chany: Étude cytochimique et ultra-structurale de cellules infectées par le virus K du rat et le virus H-1. C. R. Acad. Sci. (Paris)257, 1566–1569 (1963).Google Scholar
  3. 3.
    Cartwright, S. F., M. Lucas, andK. A. Huck: A small hemagglutinating porcine DNA virus. I. Isolation and properties. J. comp. Path.79, 371–377 (1969).Google Scholar
  4. 4.
    Hallauer, C., G. Kronauer, andG. Siegl: Parvoviruses as contaminants of permanent human cell lines. I. Virus isolations from 1960–1970. Arch. ges. Virusforsch.35, 80–90 (1971)Google Scholar
  5. 5.
    Hallauer, C, G.Siegl, and G.Kronauer: Parvoviruses as contaminants of permanent human cell lines. III. The biologic properties of the isolated viruses. Arch. ges. Virusforsch. 1972, in press.Google Scholar
  6. 6.
    Hampton, E. G.: Viral antigen in rat embryo cultures infected with the H-1 virus isolated from transplantable human tumors. Cytochemical studies. Cancer Res.24, 1534–1543 (1964).Google Scholar
  7. 7.
    Hampton, E. G.: H-1 virus growth in synchronized rat embryo cells. Canad. J. Microbiol.16, 266–268 (1970).Google Scholar
  8. 8.
    Mahnel, H., andG. Siegl: Ultrahistologische Untersuchungen über die Vermehrung eines Adenovirus vom Schwein in Zellkulturen. Zbl. Bakt. I. Abt. Orig.206, 149–159 (1969).Google Scholar
  9. 9.
    Mayor, H. D., andE. L. Jordan: Electron microscopic study of the rodent “Picodnavirus” X-14. Exp. molec. Path.5, 580–589 (1966).Google Scholar
  10. 10.
    Mayor, H. D., andM. Ito: The early detection of picodnavirus X-14 by immunofluorescenco. Proc. Soc. exp. Biol. (N.Y.)129, 684–686 (1968).Google Scholar
  11. 11.
    Mayr, A., P. A. Bachmann, G. Siegl, H. Mahnel, andB. E. Shefft: Characterization of a small porcine DNA virus. Arch. ges. Virusforsch.25, 38–51 (1968).Google Scholar
  12. 12.
    Portella, O. B.: Hemadsorption and related studies on the hamster osteolytic virus. Arch. ges. Virasforsch.14, 277–305 (1963).Google Scholar
  13. 13.
    Reynolds, E. S.: The use of leadcitrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208 (1963).Google Scholar
  14. 14.
    Siegl, G., C. Hallauer, A. Novak, andG. Kronauer: Parvoviruses as contaminants of permanent human cell lines. II. Physicochemical properties of the isolated viruses. Arch. ges. Virasforsch.35, 91–103 (1971).Google Scholar
  15. 15.
    Stempak, J. G., andR. T. Ward: An improved staining method for electron microscopy. J. Cell Biol.22, 697 (1964).Google Scholar
  16. 16.
    Tennant, R. W., K. R. Layman, andR. H. Hand, Jr.: Effect of cell physiological state on infection by rat virus. J. Virol.4, 872–878 (1969).Google Scholar
  17. 17.
    Tennant, R. W., andR. E. Hand, Jr.: Requirement of cellular synthesis for Kilham rat virus. Virology42, 1054–1063 (1970).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • G. Siegl
    • 1
  • C. Hallauer
    • 1
  • A. Novak
    • 1
  1. 1.Institute of Hygiene and Medical MicrobiologyUniversity of BernSwitzerland

Personalised recommendations