Parvoviruses as contaminants of permanent human cell lines
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The multiplication of KBSH-virus in KB cells has been investigated by means of immunofmorescence, histological, and ultrahistological techniques.
According to the results of immunofluorescence studies virus synthesis starts within the cytoplasm of an infected cell after a lag period of 5–6 hours. At that time viral antigens could be detected in the perinuclear region. During the following 6 to 8 hours increasing amounts of demonstrable antigens appeared within the nucleus and, due to the formation of brightly staining intranuclear inclusions paralleled by the reappearance of fluorescing granules in the cytoplasm, it had to be concluded that virus synthesis was completed within the nucleus after 14–20 hours following infection.
The formation of intranuclear inclusions could be readily demonstrated by means of H & E staining. At the ultrahistological level, affected nuclei showed severe signs of structural degeneration and were frequently filled with large arrays of both empty and full virus particles.
Regarding the relationship between the mitotic index of the cell monolayer at the time infection occurred and the outcome of virus replication, there was strong evidence that synthesis of KBSH-virus is favoured by cells undergoing active growth. In cells of limited physiologic activity virus replication was found to extend for more than 24 hours.
KeywordsVirus Replication Virus Particle Cell Monolayer Mitotic Index Human Cell Line
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