Molecular Breeding

, Volume 1, Issue 2, pp 165–179

Saccharum spontaneum L. ‘SES 208’ genetic linkage map combining RFLP- and PCR-based markers

  • Jorge da Silva
  • Rhonda J. Honeycutt
  • William Burnquist
  • Salah M. Al-Janabi
  • Mark E. Sorrells
  • Steven D. Tanksley
  • Bruno W. S. Sobral
Article

Abstract

A 527 marker linkage map ofSaccharum spontaneum L. ‘SES 208’ (2n = 64) was established by analyzing 208 single-dose (SD) arbitrarily primed PCR polymorphisms, 234 SD RFLPs, 41 double-dose (DD) and one triple-dose (TD) polymorphisms. A map hypothesis constructed using these markers (minimum LOD = 4.00,θ = 0.25 M) had 64 linkage groups with 13 SD, nine DD, and one TD markers unlinked. Eight chromosome homology groups were identified by using DD fragments as well as SD RFLPs that identified more than one linkage group. Linkages in repulsion phase were absent from the map, as found in two previous genetic studies of this species. Together, these data demonstrate that SES 208 displayed polysomic segregation, a genetic behavior typical of autopolyploid species. As with previous studies, it was concluded that SES 208 behaved like an auto-octoploid, which was also in agreement with the number of homology groups observed. Aχ2 was used to test whether the 527 markers were randomly distributed throughout the genome: both arbitrarily primed PCR markers and RFLPs had a distribution that was statistically indistinguishable from random. The integrated arbitrarily primed PCR-RFLP map had a predicted genomic coverage of 93% (considering only 442 SD polymorphisms) and an average interval between markers of 6 cM. SD markers were used to estimate the genome size of SES 208 at ca. 33 00 cM.

Key words

arbitrarily primed PCR polyploidy polysomic inheritance RAPD markers single-dose fragments sugarcane 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Jorge da Silva
    • 2
    • 3
  • Rhonda J. Honeycutt
    • 1
  • William Burnquist
    • 3
  • Salah M. Al-Janabi
    • 1
  • Mark E. Sorrells
    • 2
  • Steven D. Tanksley
    • 2
  • Bruno W. S. Sobral
    • 1
  1. 1.California Institute of Biological ResearchLa JollaUSA
  2. 2.Department of Plant Breeding and BiometryCornell UniversityIthacaUSA
  3. 3.Copersucar Technology CenterPiracicabaBrazil
  4. 4.Agronomy DepartmentBaghdad Institute of Agricultural TechnologyIraq

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