, Volume 59, Issue 2, pp 368–373 | Cite as

Polychromatic staining of plant cell walls by toluidine blue O

  • T. P. O'Brien
  • N. Feder
  • M. E. McCully


  1. 1.

    The polychromatic staining of plant cell walls by toluidine blue O is described and illustrated.

  2. 2.

    The effects of various common fixatives and the effects of the pH of the staining solution are evaluated.

  3. 3.

    Simple and rapid procedures are described for preparing stained temporary mounts of fresh material, or permanent mounts of embedded and sectioned material.

  4. 4.

    The relationship between the polychromatic staining observed and the lignification of the walls is discussed.



Cell Wall Plant Cell Toluidine Toluidine Blue Plant Cell Wall 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Barghoorn, E. S., 1948: Sodium chlorite as an aid in paleobotanical and anatomical study of plant tissues. Science107, 480–481.Google Scholar
  2. Bergeron, J. A., and M. Singer. 1958: Metachromasy: an experimental and theoretical reevaluation. J. Biophys. Biochem. Cytol.4, 433–457.Google Scholar
  3. Czaja, A. Th., 1930: Untersuchungen über metachromatische Färbungen von Pflanzengeweben. 1. Substantive Farbstoffe. Planta11, 582–626.Google Scholar
  4. —, 1934: Untersuchungen über nietachromatische Färbungen von Pflanzengeweben. II. Basische Farbstoffe. Planta21, 531–601.Google Scholar
  5. Jensen, W. A., 1962: Botanical histochemistry. p. 197. W. H. Freeman & Co.Google Scholar
  6. Johansen, D. A., 1940: Plant microtechnique, p. 194. McGraw-Hill Book Co.Google Scholar
  7. Sidman, R. L., P. A. Mott1a, and N. Feder. 1961: Improved polyester wax embedding for histology. Stain Tech.36, 279–284.Google Scholar
  8. Spurr, A. R., 1954: Polyvinyl alcohol with cadmium iodide and fructose as an aqueous mounting medium. Stain Tech.29, 301–313.Google Scholar

Copyright information

© Springer-Verlag 1964

Authors and Affiliations

  • T. P. O'Brien
    • 1
  • N. Feder
    • 1
  • M. E. McCully
    • 1
  1. 1.The Biological LaboratoriesHarvard, CambridgeUSA

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