Molecular Breeding

, Volume 1, Issue 4, pp 397–410

The inheritance and chromosomal localization of AFLP markers in a non-inbred potato offspring

  • Herman J. van Eck
  • Jeroen Rouppe van der Voort
  • Jan Draaistra
  • Peter van Zandvoort
  • Ellen van Enckevort
  • Bart Segers
  • Johan Peleman
  • Evert Jacobsen
  • Johannes Helder
  • Jaap Bakker
Article

Abstract

AFLPTM is a new technique to generate large numbers of molecular markers for genetic mapping. The method involves the selective amplification of a limited number of DNA restriction fragments out of complex plant genomic DNA digests using PCR. With six primer combinations 264 segregating AFLP amplification products were identified in a diploid backcross population from non-inbred potato parents. The identity of an AFLP marker was specified by the primer combination of the amplification product and its size estimated in bases. The segregating AFLP amplification products were mapped by using a mapping population with 217 already known RFLP, isozyme and morphological trait loci. In general, the AFLP markers were randomly distributed over the genome, although a few clusters were observed. No indications were found that AFLP markers are present in other parts of the genome than those already covered by RFLP markers. Locus specificity of AFLP markers was demonstrated because equally sized amplification products segregating from both parental clones generally mapped to indistinguishable maternal and paternal map positions. Locus specificity of AFLP amplification products will allow to establish the chromosomal identity of linkage groups in future mapping studies.

Since AFLP technology is a multi-locus detection system, it was not possible to identify the AFLP alleles which belong to a single AFLP locus. The consequences of a genetic analysis based on single alleles, rather than on loci with two or more alleles on mapping studies using progenies of non-inbred parents are discussed.

Key words

AFLP marker allelism allogamous linkage map Solanum tuberosum 

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Herman J. van Eck
    • 1
    • 4
  • Jeroen Rouppe van der Voort
    • 2
    • 4
  • Jan Draaistra
    • 2
    • 4
  • Peter van Zandvoort
    • 2
    • 4
  • Ellen van Enckevort
    • 2
    • 4
  • Bart Segers
    • 3
    • 4
  • Johan Peleman
    • 3
    • 4
  • Evert Jacobsen
    • 1
    • 4
  • Johannes Helder
    • 2
    • 4
  • Jaap Bakker
    • 2
    • 4
  1. 1.Department of Plant BreedingWageningenThe Netherlands
  2. 2.Department of NematologyWageningenThe Netherlands
  3. 3.Keygene N.V.WageningenThe Netherlands
  4. 4.The Graduate School of Experimental Plant SciencesWageningen Agricultural UniversityThe Netherlands

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