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International Journal of Legal Medicine

, Volume 107, Issue 1, pp 34–36 | Cite as

Swiss population data on three tetrameric short tandem repeat loci — VWA, HUMTH01, and F13A1 —derived using multiplex PCR and laser fluorescence detection

  • M. N. Hochmeister
  • J. M. Jung
  • B. Budowle
  • U. V. Borer
  • R. Dirnhofer
Original Article

Abstract

Allele and genotype frequencies for 3 tetrameric short tandem repeat loci VWA, HUMTHO1, and F13A1 were determined in a Swiss population sample using multiplex PCR and subsequent electrophoresis in DNA sequencing gels processed by automated laser fluorescence detection. The technique allows single base pair resolution and rapid typing, with a concomitant reduction in the potential for human transcriptional typing errors. All loci meet Hardy-Weinberg expectations. In addition, there is little evidence for association of alleles among the 3 loci. The allelic frequency data can be used in forensic analyses and paternity tests to estimate the frequency of a multiple STR locus DNA profile in the Swiss population.

Key words

PCR Multiplex VWA HUMTHO1 F13A1 Fluorescence detection Hardy-Weinberg expectations Short tandem repeat loci 

Zusammenfassung

An einer Schweizer Populationsstichprobe wurden mittels Multiplex-PCR und automatisierter Fluoreszenzdetektion (Applied Biosystem 373A DNA Sequencer) die Allel- und Genotypfrequenzen der Short tandem repeat (STR) loci VWA, HUMTHO1 und F13A1 bestimmt. Die Daten wurden umfangreichen statistischen Analysen unterzogen. Alle Loci erfüllen die Hardy-Weinberg-Kriterien. Die ermittelten Daten erlauben die Abschätzung der Häufigkeit des Vorkommens eines Multiplex-STR-Profils in der Schweizer Bevölkerung zur Anwendung in forensischen DNA-Analysen und Vaterschaftsuntersuchungen.

Schlüsselwörter

PCR Multiplex2 VWA HUMTHO1 F13A1 Fluoreszenzdetektion Hardy-Weinberg-Gleichgewicht Short tandem repeat loci 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. N. Hochmeister
    • 1
  • J. M. Jung
    • 2
  • B. Budowle
    • 2
  • U. V. Borer
    • 1
  • R. Dirnhofer
    • 1
  1. 1.Department of Forensic Medicine, Institut für RechtsmedizinUniversity of BernBernSwitzerland
  2. 2.Forensic Science Research and Training CenterFBI AcademyQuanticoUSA

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