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Immunogenetics

, Volume 40, Issue 5, pp 360–369 | Cite as

Structure, chromosomal localization, and expression of 12 genes of the MAGE family

  • Etienne De Plaen
  • Catia Traversari
  • José J. Gaforio
  • Jean-Pierre Szikora
  • Charles De Smet
  • Francis Brasseur
  • Pierre van der Bruggen
  • Bernard Lethé
  • Christophe Lurquin
  • Patrick Chomez
  • Olivier De Backer
  • Thierry Boon
  • Karen Arden
  • Webster Cavenee
  • Robert Brasseur
Original Paper

Abstract

We reported previously that human geneMAGE-1 directs the expression of a tumor antigen recognized on a melanoma by autologous cytolytic T lymphocytes. Probing cosmid libraries with aMAGE-1 sequence, we identified 11 closely related genes. The analysis of hamster-human somatic cell hybrids indicated that the 12MAGE genes are located in the q terminal region of chromosome X. LikeMAGE-1, the 11 additionalMAGE genes have their entire coding sequence located in the last exon, which shows 64%-85% identity with that ofMAGE-1. The coding sequences of theMAGE genes predict the same main structural features for allMAGE proteins. In contrast, the promoters and first exons of the12 MAGE genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The expression of eachMAGE gene was evaluated by reverse transcription and polymerase chain reaction amplification. Six genes of theMAGE family includingMAGE-1 were found to be expressed at a high level in a number of tumors of various histological types. None was expressed in a large panel of healthy tissues, with the exception of testis and placenta.

Keywords

Melanoma Polymerase Chain Reaction Amplification Chromosomal Localization Tumor Antigen Healthy Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Etienne De Plaen
    • 1
    • 2
  • Catia Traversari
    • 1
    • 2
  • José J. Gaforio
    • 1
    • 2
  • Jean-Pierre Szikora
    • 1
    • 2
  • Charles De Smet
    • 1
    • 2
  • Francis Brasseur
    • 1
    • 2
  • Pierre van der Bruggen
    • 1
    • 2
  • Bernard Lethé
    • 1
    • 2
  • Christophe Lurquin
    • 1
    • 2
  • Patrick Chomez
    • 1
    • 2
  • Olivier De Backer
    • 1
    • 2
  • Thierry Boon
    • 1
    • 2
  • Karen Arden
    • 3
  • Webster Cavenee
    • 3
  • Robert Brasseur
    • 4
  1. 1.Ludwig Institute for Cancer Research, Brussels BranchBelgium
  2. 2.Cellular Genetics UnitUniversity Catholique de LouvainBelgium
  3. 3.Ludwig Institute for Cancer Research and Department of MedicineUniversity of California-San DiegoCALa JollaUSA
  4. 4.Laboratoire de Chimie Physique des Macromolecules aux Interfaces CP206-2University Libre de BruxellesBrusselsBelgium

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