Depletion of the Ca++-dependent releasable pool of glutamate in striatal synaptosomes associated with dendrotoxin-induced potassium channel blockade

  • L. Barbeito
  • J. Siciliano
  • F. Dajas
Full Papers

Summary

The presynaptic actions of the potassium channel blocker Dendrotoxin (DTX) on the Ca+2-dependent release of endogenous glutamate (GLU) and aspartate (ASP) have been tested in synaptosome-enriched preparations from rat striatum.

24 hours after the intrastriatal administration of DTX the K+-evoked release of GLU and ASP from the striatal synaptosomes was decreased by 40–45%. No changes in the total synaptosomal content of the amino acids were observed. Superfusion of immobilized synaptosomes with DTX or 4-aminopyridine resulted in a dose-dependent increase in the basal outflow of GLU and ASP. The release of GLU stimulated by DTX was Ca+2-dependent and was not abolished by supervising the synaptosomes with 50 μM D-ASP. Moreover, continous superfusion of DTX (7 μM) to synaptosomes almost completely dumped the subsequent release of GLU and ASP stimulated by 20mM K+. It is concluded that blockade of presyanptic K+ channels by DTX leads to a massive release of the transmitter pool of GLU (and possible also ASP) from isolated nerve terminals and to a depletion of the amino acid releasable pool.

Keywords

Glutamate release K+ channels Dendrotoxin synaptosomes 

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • L. Barbeito
    • 1
    • 2
  • J. Siciliano
    • 1
    • 2
  • F. Dajas
    • 1
    • 2
  1. 1.Neurochemistry DivisionIIBCEUruguay
  2. 2.Histology DepartmentFaculty of MedicineMontevideoUruguay

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