Microchimica Acta

, Volume 120, Issue 1–4, pp 231–242 | Cite as

Biocatalysis and biorecognition in nonaqueous media. Some perspectives in analytical biochemistry

  • Lorenzo Braco
Original Papers


Biocatalysis and, to a lesser extent, biorecognition in non-aqueous media (including organic solvents as well as supercritical fluids and gases) constitute at present an exciting research area which has already demonstrated its biotechnological potential in numerous, varied applications. Less attention, however, has been paid to its analytical possibilities, even though many advantages have been postulated and a wide range of poorly water-soluble analytes are present in samples (or waste materials) from food and drink, petrochemical, pharmaceutical, military and other industries. The main approaches, developed in recent years to exploit the use of enzymes, antibodies or antibody mimics in water-restricted environments for analytical purposes, as well as possible future directions are briefly discussed.

Key words

non-aqueous enzymology antibodies flow-injection analysis biosensors molecular imprinting review 


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Lorenzo Braco
    • 1
  1. 1.Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias BiológicasUniversidad de ValenciaBurjassot, ValenciaSpain

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