Microchimica Acta

, Volume 128, Issue 3–4, pp 127–143 | Cite as

Oriented immobilization of proteins

  • Srivatsa V. Rao
  • Kimberly W. Anderson
  • Leonidas G. Bachas
Fundamental Review
Received:

Abstract

Immobilized enzymes have found numerous applications in analytical, clinical, environmental and industrial chemistry. However, in most cases, immobilization leads to partial or total loss of activity. It is widely believed that the loss in activity is due to attachment of proteins on the immobilization support through several amino acid residues. This results in a random orientation of the immobilized protein and in increased structural deformation due to multi-point attachment. Several researchers have explored ways to orient proteins on surfaces, such that orderly organization, single point attachment and accessibility of the active site (or binding site) are possible. This article reviews the various approaches available to achieve oriented immobilization of proteins and its applications in several disciplines.

Key words

protein immobilization site-specific immobilization site-directed mutagenesis carbohydrate-mediated immobilization protein orientation 
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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • Srivatsa V. Rao
    • 1
  • Kimberly W. Anderson
    • 1
  • Leonidas G. Bachas
    • 2
  1. 1.Department of Chemical and Materials EngineeringLexingtonUSA
  2. 2.Department of ChemistryUniversity of KentuckyLexingtonUSA

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