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Microchimica Acta

, Volume 130, Issue 4, pp 267–272 | Cite as

Characterization of a urea optical sensor based on polypyrrole

  • Susana de Marcos
  • Rubén Hortigüela
  • Javier Gatbán
  • Juan R. Castillo
  • Otto S. Wolfbeis
Original Papers

Abstract

A new optical sensor for urea determination is presented. It is based on the enzymatic reaction with urease, which is first photoimmobilized with polyacrylamide onto a chemically polymerized polypyrrole (PPy) film. The main advantage of this sensor is that no indicator dye or pH indicator is needed, because PPy itself acts as the support and the indicator. These PPy films show an absorbance spectrum in the near IR range which is pH dependent. The variation of absorbance is thus directly related to the change of pH caused during the enzymatic reaction, which is also dependent on the urea concentration. The linear range of the sensor is from 0.06 to 1 M of urea, which is the common level of urea concentration found in blood and urine samples.

Key words

optical sensor urea urease polypyrrole 

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References

  1. [1]
    M. H. Gil, A. P. Piedade, S. Alegret, J. Alonso, E. Martinez, A. Orellana.Biosensors Bioelectron. 1992,7, 645.Google Scholar
  2. [2]
    N. Das, P. Prabhakar, M. Kayastha, R. C. Srivastava,Biotech. Bioeng. 1997,54, 329.Google Scholar
  3. [3]
    D. Z. Shen, Q. Kang, X. Y. Wang,Mikrochim. Acta 1997,126, 267.Google Scholar
  4. [4]
    S. B. Adeloju, S. J. Shaw, G. G. Wallace,Anal. Chim. Acta 1993,281, 611.Google Scholar
  5. [5]
    S. Luo, D. R. Walt,Anal. Chem. 1989,61, 1069.Google Scholar
  6. [6]
    C. Stamm, K. Seiler, W. Simon,Anal. Chim. Acta 1993,282, 229.Google Scholar
  7. [7]
    S. de Marcos, O. S. Wolfbeis,Sensors Mat. 1997,9, 253.Google Scholar
  8. [8]
    S. de Marcos, O. S. Wolfbeis,Anal. Chim. Acta 1996,334, 149.Google Scholar

Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Susana de Marcos
    • 1
  • Rubén Hortigüela
    • 1
  • Javier Gatbán
    • 1
  • Juan R. Castillo
    • 1
  • Otto S. Wolfbeis
    • 2
  1. 1.GEAS (Analytical Spectrometry and Sensors Group), Analytical Chemistry Department, Faculty of SciencesUniversity of ZaragozaZaragozaSpain
  2. 2.Instiute of Analytical Chemistry, Chemo- and BiosensorsUniversity of RegensburgRegensburgGermany

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