Microchimica Acta

, Volume 126, Issue 3–4, pp 203–206 | Cite as

Determination of pefloxacin in serum and pharmaceutical forms by derivative spectrophotometry

  • Predrag Djurdjeviċ
  • Milena Jelikiċ-Stankov
  • Zoran Miliċeviċ
Original Papers
Received:
Revised:

Abstract

A method for the direct determination of pefloxacin in serum and pharmaceutical forms (tablets and ampoules) has been developed, based on the use of second-order derivative ultraviolet spectra. Spectrophotometric assay of pefloxacin in tablets and ampoules was carried out in 0.1 mol/L NaOH, while in serum it was performed in 0.1 mol/L NaOH with the addition of sodium dodecylsulfate, in 337–347 nm wavelength range. Linear calibration curves were obtained in the concentration ranges 2–30μg/mL pefloxacin for tablets and ampoules and 0.12–5 μg/ mL for serum samples. Relative error of determination, as criterion for accuracy, was less than 1%, while the precision was better than 4 ng/ml. The minimum detectable concentration of pefloxacin in serum was 15 ng/mL.

Key words

pefloxacin serum pharmaceutical forms derivative spectrophotometry 
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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Predrag Djurdjeviċ
    • 1
  • Milena Jelikiċ-Stankov
    • 2
  • Zoran Miliċeviċ
    • 3
  1. 1.Chemistry DepartmentFaculty of ScienceKragujevacYugoslavia
  2. 2.Faculty of PharmacyBelgradeYugoslavia
  3. 3.Institute for Plant and Environment ProtectionBelgradeYugoslavia

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