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Archiv für die gesamte Virusforschung

, Volume 37, Issue 4, pp 297–307 | Cite as

An investigation of the replication of coronaviruses in suspension cultures of L132 cells

  • A. F. Bradburne
Article

Summary

A system is described for the study of the multiplication of two coronaviruses, both of human origin. Low efficiences of adsorption and infectious centre formation were observed. The viruses were found to have a 4–6 hour eclipse phase in L 132 cells at 33° C. Both viruses were completely inhibited by raising the incubation temperature to 39° C, and there was also a depression in the yield of virus at 30° C compared with control cultures maintained at 33° C. “Shift” experiments have indicated that the high temperature-sensitive event occurred fairly late on in the replicative cycle, but the time could not be accurately determined.

Keywords

Infectious Disease Suspension Culture Control Culture Incubation Temperature L132 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • A. F. Bradburne
    • 1
  1. 1.Common Cold Research Unit, Clinical Research CentreHarvard HospitalSalisburyEngland

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