Archiv für die gesamte Virusforschung

, Volume 37, Issue 4, pp 297–307 | Cite as

An investigation of the replication of coronaviruses in suspension cultures of L132 cells

  • A. F. Bradburne


A system is described for the study of the multiplication of two coronaviruses, both of human origin. Low efficiences of adsorption and infectious centre formation were observed. The viruses were found to have a 4–6 hour eclipse phase in L 132 cells at 33° C. Both viruses were completely inhibited by raising the incubation temperature to 39° C, and there was also a depression in the yield of virus at 30° C compared with control cultures maintained at 33° C. “Shift” experiments have indicated that the high temperature-sensitive event occurred fairly late on in the replicative cycle, but the time could not be accurately determined.


Infectious Disease Suspension Culture Control Culture Incubation Temperature L132 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Almeida, June D., andD. A. J. Tyrrell: The morphology of three previously uncharacterized respiratory viruses that grow in organ cultures. J. Virol.1, 175 (1967).Google Scholar
  2. Becker, W. B., K. McIntosh, J. H. Dees, andR. M. Chanock: Morphogenesis of avian infectious bronchitis virus and a related human virus (Strain 229 E). J. Virol.1, 1019 (1967).PubMedGoogle Scholar
  3. Berry, D. M., J. G. Cruickshank, H. P. Chu, andR. J. H. Wells: The structure of infectious bronchitis virus. Virology23, 403 (1964).PubMedGoogle Scholar
  4. Bradburne, A. F., M. L. Bynoe, andD. A. J. Tyrrell: Effects of a “new” human respiratory virus in volunteers. Brit. med. J.3, 767 (1967).PubMedGoogle Scholar
  5. Bradburne, A. F., andD. A. J. Tyrrell: The propagation of “coronaviruses” in tissue-culture. Arch. ges. Virusforsch.28, 133 (1969).PubMedGoogle Scholar
  6. Bradburne, A. F.: Antigenic relationships amongst coronaviruses. Arch. ges. Virusforsch.31, 352 (1970).PubMedGoogle Scholar
  7. Chany, C., andF. Robbe-Maridor: Enhancing effect of the murine sarcoma virus (MSV) on the replication of the mouse hepatitis virus (MHV)in vitro. Proc. Soc. exp. Biol. (N. Y.)131, 30 (1970).Google Scholar
  8. Crick, Joan., B. Cartwright, andF. Brown: Studies on the interference phenomenon in vesicular stomatitis replication. Arch. ges. Virusforsch.27, 235 (1969).Google Scholar
  9. David-Ferreira, J. F., andR. H. Manaker: An electron microscope study of the development of a mouse hepatitis virus in tissue culture cells. J. Cell Biol.24, 57 (1965).PubMedGoogle Scholar
  10. Davis, E. V.: Continuous cultivation of isogenous cell lines from the human embryo. Fed. Proc.19, 57 (1960).Google Scholar
  11. Hamre, D., D. A. Kindig, andJ. Mann: Growth and intracellular development of a new respiratory virus. J. Virol.1, 810 (1967).PubMedGoogle Scholar
  12. Hamre, D., andJ. J. Procknow: A new virus isolated from the human respiratory tract. Proc. Soc. exp. Biol. (N. Y.)121, 190 (1966).Google Scholar
  13. Kapikian, A. Z., H. D. James, Sarah J. Kelly, Jane H. Dees, H. C. Turner, K. McIntosh, H. W. Kim, R. H. Parrott, M. M. Vincent, andR. M. Chanock: Isolation from man of “avian infectious bronchitis virus-like” viruses (Coronaviruses) similar to 229E virus, with some epidemiological observations. J. infect. Dis.119, 282 (1969).PubMedGoogle Scholar
  14. Lwoff, A.: Death and transfiguration of a problem. Bact. Rev.33, 390 (1969).PubMedGoogle Scholar
  15. McIntosh, K., Jane H. Dees, W. B. Becker, A. Z. Kapikian, andR. M. Chanock: Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. nat. Acad. Sci. (Wash.)57, 933 (1967).Google Scholar
  16. McIntosh, K., A. Z. Kapikian, Kathleen A. Hardison, Janet W. Hartley, andR. M. Chanock: Antigenic relationships among the coronaviruses of man and between human and animal coronaviruses. J. Immunol.102, 1109 (1969).PubMedGoogle Scholar
  17. Nature (Lond.) Coronaviruses.220, 650 (1968).Google Scholar
  18. Stott, E. J., andG. F. Heath: Factors affecting the growth of Rhinovirus 2 in suspension cultures of L 132 cells. J. gen. Virol.6, 15 (1970).PubMedGoogle Scholar
  19. Tajima, M.: Morphology of transmissible gastroenteritis virus of pigs: a possible member of coronaviruses. Arch. ges. Virusforsch.29, 105 (1970).PubMedGoogle Scholar
  20. Tyrrell, D. A. J., andM. L. Bynoe: Cultivation of a novel type of common cold virus in organ cultures. Brit. med. J.i, 1467 (1965).Google Scholar
  21. Tyrrell, D. A. J., M. L. Bynoe, andB. Hoorn: Cultivation of “difficult” viruses from patients with common colds. Brit. med. J.i, 606 (1968).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • A. F. Bradburne
    • 1
  1. 1.Common Cold Research Unit, Clinical Research CentreHarvard HospitalSalisburyEngland

Personalised recommendations