Temperature-and UV-light resistance of rubella virus infectivity
- 43 Downloads
- 2 Citations
Summary
The thermal and UV-light sensitivity of a laboratory-adapted rubella virus (strain Wright) was assayed quantitatively and by electron microscopic observation. As judged by the appearence of cytopathic effects in cultures of African green monkey kidney cells (Vero) and/or by the presence of rubella virus particles in electron microscopic preparations, infectivity was found to withstand inactivation temperatures up to 70° C for 30 minutes. Infectious tissue culture fluid treated by UV-irradiation for 60 minutes also produced cytopathic effects in Vero cells. Ultrastructurally, however, only a few rubella virus-like particles could be detected. Virus suspensions filtered through 0.22 μ membrane filters prior to heat-or UV-treatment failed to reveal residual infectivity. Some of the possible mechanisms responsible for this marked thermal and UV-resistance of the rubella virus strain used are discussed.
Keywords
Vero Cell Rubella Cytopathic Effect Monkey Kidney African Green MonkeyPreview
Unable to display preview. Download preview PDF.
References
- 1.Parkman, P. D., E. L. Buescher, M. S. Artenstein, J. M. McCown, F. K. Mundon, andA. D. Druzd: Studies on rubella. I. Properties of the virus. J. Immunol.93, 595–607 (1964).Google Scholar
- 2.Fabiyi, A., J. L. Sever, N. Ratner, andB. Caplan: Rubella virus: Growths characteristics and stability of infectious virus and complement-fixing antigen. Proc. Soc. exp. Biol. (N.Y.)122, 392–396 (1966).Google Scholar
- 3.Parkman, P. D., E. L. Buescher, andM. S. Artenstein: Recovery of rubella virus from army recruits. Proc. Soc. exp. Biol. (N.Y.)111, 225–230 (1962).Google Scholar
- 4.Sever, J. L., G. M. Schiff, andR. G. Traub: Rubella virus. J. Amer. med. Ass.182, 663–671 (1962).Google Scholar
- 5.Sigurdardottir, B., K. F. Given, K. R., Rozee, andA. J. Rhodes: Association of virus with cases of rubella studies in Toronto: propagation of the agent and transmission to monkeys. Canad. med. Ass. J.88, 128–132 (1963).Google Scholar
- 6.Weller, T. H., andF. A. Neva: Propagation in tissue culture of cytopathic agents from patients with rubella-like illness. Proc. Soc. exp. Biol. (N.Y.)111, 215–225 (1962).Google Scholar
- 7.Parkman, P. D.: Biological characteristics of rubella virus. Arch. ges. Virusforsch.16, 401–411 (1965).Google Scholar
- 8.Norrby, E.: Rubella virus. Monographs in Virology, No. 7, pp. 115–174, Wien-New York: Springer Verlag, 1969.Google Scholar
- 9.Rütsch, A.: Eigenschaften der ultravioletten Strahlen und ihre Wirkung auf Mikroorganismen: Keimtötende TUV-Lampen. Philips: Technical Manual, 1970.Google Scholar
- 10.Fraska, I. M., andV. R. Parks: A routine technique for double-staining ultrathin sections using uranyl- and lead salts. J. Cell Biol.,25, 157–161 (1965).Google Scholar
- 11.Vaheri, A., C.-H. von Bonsdorff, T. Vesikari, T. Hovi, andP. Väänänen: Purification of rubella virus particles. J. gen. Virol.5, 39–46 (1969).Google Scholar
- 12.Frankel, J. W.: Neutralization antibody responses of guinea-pigs to inactivated rubella virus vaccine. Nature (Lond.)204, 655–656 (1964).Google Scholar
- 13.Sever, J. L., G. M. Schiff, andR. J. Huebner: Inactivated rubella virus vaccine. J. Lab. clin. Med.62, 1015 (1963).Google Scholar
- 14.Herriott, R. M.: Implications of infectious nucleic acids in disease. Progr. med. Virol., vol. 11, pp. 1–15, Basel-New York: Karger, 1969.Google Scholar
- 15.Hovi, T., andA. Vaheri: Infectivity and some physicochemical characteristics of rubella virus ribonucleic acid. Virology42, 1–8 (1970).Google Scholar