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CHEMOECOLOGY

, Volume 2, Issue 1, pp 7–14 | Cite as

Chemical defence in ladybird beetles (Coccinellidae). I. Distribution of coccinelline and individual variation in defence in 7-spot ladybirds (Coccinella septempunctata)

  • Graham J. Holloway
  • Peter W. de Jong
  • Paul M. Brakefield
  • Helene de Vos
Research papers

Summary

7-spot ladybirds secrete alkaloid (coccinelline)-rich fluid (reflex blood) from leg joints as a defence mechanism against predators. A technique is described that enables the collection and accurate quantification of reflex blood produced, and the amount of coccinelline therein. Coccinelline was found distributed throughout the body, although concentrated in the reflex blood. Reflex blood was collected from a large set of beetles at several time points. Significant variation was found among beetles in the amount of reflex blood produced (for males and for females corrected for body weight) and the coccinelline concentration of the reflex blood. The results are discussed in relation to automimicry and the maintenance of variation through energy trade-offs. The relationships between tendency to aggregate, ability to reflex bleed and the possession of aposematic coloration are also considered.

Key words

chemical defence mimicry reflex bleeding variation alkaloid coccinelline Coleoptera Coccinellidae Coccinella septempunctata 

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References

  1. Ayer WA, Browne LM (1977) The ladybug alkaloids including synthesis and biosynthesis. Heterocycles 7:685–707Google Scholar
  2. Becker WA (1967) Manual of Quantitative Genetics (3rd ed). Washington: Washington State UniversityGoogle Scholar
  3. Bell G, Koufopanou V (1986) The cost of reproduction. Pp 83–131in Dawkins R & Ridley M (eds) Oxford Surveys in Evolutionary Biology, Vol 3. New York: Oxford University PressGoogle Scholar
  4. Brakefield PM (1985a) Polymorphic Müllerian mimicry and interactions with thermal melanism in ladybirds and a soldier beetle: a hypothesis. Biol J Linn Soc 26:243–267Google Scholar
  5. Brakefield PM (1985b) Differential winter mortality and seasonal selection in the polymorphic ladybirdAdalia bipunctata (L.) in the Netherlands. Biol J Linn Soc 24:189–206Google Scholar
  6. Brower LP (1984) Chemical defence in butterflies. Pp 109–132in Vane-Wright RI & Ackery PR (eds) The Biology of Butterflies. Symp R ent Soc No 11. London: Academic PressGoogle Scholar
  7. Brower LP, Brower JVZ, Corvino JM (1967) Plant poisons in a terrestrial food chain. Proc Natl Acad Sci 57:893–898Google Scholar
  8. Brower LP, Pough FH, Meck HR (1970) Theoretical investigations of automimicry. I. Single trial learning. Proc Natl Acad Sci USA 66:1059–1066Google Scholar
  9. Cuenot L (1896) Sur la saignée réflexe et les moyens de défense de quelques insectes. Archs Zool exp gén 4:655–680Google Scholar
  10. Davidson BS, Eisner T, Witz B, Meiwald J (1989) Defensive secretions of the carabid beetlePasimachus subsulcatus. J Chem Ecol 15:1689–1697Google Scholar
  11. Dawkins R (1978) The Selfish Gene. London: Granada PublishingGoogle Scholar
  12. Dobzhansky Th, Sivertzev-Dobzhansky NP (1927) Die geographische Variabilität vonCoccinella septempunctata L. Biol Zentrallblatt 47:556–569Google Scholar
  13. Eisner T, Goetz M, Aneshansley D, Ferstandig-Arnold G, Meinwald J (1986) Defensive alkaloid in blood of Mexican bean beetle (Epilachna varivestis). Experientia 42:204–207Google Scholar
  14. Falconer DS (1981) Introduction to Quantitative Genetics (2nd ed). London: LongmanGoogle Scholar
  15. Fisher RA (1930) The Genetical Theory of Natural selection. Oxford: Oxford University PressGoogle Scholar
  16. Frazer JFD, Rothschild M (1960) Defence mechanisms in warningly coloured moths and other insects. Proc 11th int Congr Ent Symp 4:249–256Google Scholar
  17. Gibson DO (1974) Batesian mimicry without distastefulness. Nature 250:77–79Google Scholar
  18. Guilford T (1988) The evolution of conspicuous coloration. Amer Nat Suppl. 131:S7-S21Google Scholar
  19. Guilford T, Nicol C, Rothschild M, Moore BP (1987) The biological roles of pyrazines: evidence for a warning odour function. Biol J Linn Soc 31:113–128Google Scholar
  20. Happ GM, Eisner T (1961) Hemorrhage in a coccinellid beetle and its repellent effect on ants. Science 134:329–331Google Scholar
  21. Hemptinne JL (1988) Ecological requirements for hibernatingPropylea quatuordecimpuntata (L.) andCoccinella septempunctata (Col: Coccinellidae). Entomophaga 33:505–515Google Scholar
  22. Hodek I (1973) Biology of Coccinellidae. Prague: The Hague and AcademiaGoogle Scholar
  23. Hollande CH (1911) L'autohémorrhée ou le rejet du sang chez les insectes (toxicologie du sang). Archs Anat microsc 13:171–318Google Scholar
  24. Holloway GJ, Povey SR, Sibly RM (1990a) The effect of new environment on adapted genetic architecture. Heredity 64:323–330Google Scholar
  25. Holloway GJ, Sibly RM, Povey SR (1990b) Evolution in toxin stressed environments. Funct Ecol 4:289–294Google Scholar
  26. de Jong PW, Holloway GJ, Brakefield PM, de Vos H (1991) Chemical defence in ladybird beetles (Coccinellidae). II. Amount of reflex fluid, the alkaloid adaline and individual variation in defence in 2-spot ladybirds (Adalia bipunctata). Chemoecology 2:15–19Google Scholar
  27. Kay D, Rothschild M, Aplin R (1969) Particles present in the haemolymph and defensive secretions of insects. J Cell Sci 4:369–379Google Scholar
  28. Luckinbill LS, Arking R, Clare MJ, Cirocco WC, Buck SA (1984) Selection for delayed senescence inDrosophila melanogaster. Evolution 38:996–1003Google Scholar
  29. Majerus M, Kearns P (1989) Ladybirds. Naturalist Handbooks 10. Slough, England: Richmond Publishing Co LtdGoogle Scholar
  30. Malcolm SB (1990) Mimicry: status of a classical evolutionary paradigm. TREE 5:57–62Google Scholar
  31. Malcolm SB, Brower LP (1989) Evolutionary and ecological implications of cardenolide sequestration in the monarch butterfly. Experientia 45:284–295Google Scholar
  32. Marples NM (1990) The influence of predation on ladybird colour pattern. Ph D thesis: University of Wales College of CardiffGoogle Scholar
  33. Marples NM, Brakefield PM, Cowie RJ (1989) Differences between the 7-spot and 2-spot ladybird beetles (Coccinellidae) in their toxic effects on a bird predator. Ecol Entomol 14:79–84Google Scholar
  34. Maynard-Smith J (1982) Evolution and the Theory of Games. Cambridge: Cambridge University PressGoogle Scholar
  35. Meuller RH, Thompson ME, DiPardo RM (1984) Stereo-and regioselective total synthesis of the hydropyrido [2, 1, 6—de]quinolizine ladybug defensive alkaloids. J Org Chem 49:2217–2231Google Scholar
  36. Møller H, Smith RH, Sibly RM (1989) Evolutionary demography of a bruchid beetle. I. Quantitative genetic analysis of the female life history. Funct Ecol 3:673–681Google Scholar
  37. Pasteels JM, Deroe C, Tursch B, Braekman JC, Daloze D, Hootele C (1973) Distribution et activites des alcaloides defensifs des Coccinellidae. J Insect Physiol 19:1771–1784Google Scholar
  38. Pough FH, Brower LP, Meck HR, Kessell SR (1973) Theoretical investigations of automimicry: multiple trial learning and the palatability spectrum. Proc Natl Acad Sci 70:2261–2265Google Scholar
  39. Rose MR (1982) Antagonistic pleiotropy, dominance, and genetic variation. Heredity 48:63–78Google Scholar
  40. Rose MR, Charlesworth B (1981) Genetics of life history inDrosophila melanogaster. I. Sib analysis of adult females. Genetics 97:173–186Google Scholar
  41. Rothschild M (1961) Defensive odours and Mullerian mimicry among insects. Trans R Soc Lond 113:101–121Google Scholar
  42. Rothschild M (1985) British aposematic Lepidoptera. Pp 9–62in Heath J (ed) The Moths and Butterflies of Great Britain and Ireland, Vol. 2. Cossidae to Helionidae. Colchester, England: Harley BooksGoogle Scholar
  43. Sheppard PM, Turner JRG, Brown KS, Benson WW, Singer MC (1985) Genetics and the evolution of Mullerian mimicry inHeliconius butter-flies. Phil Trans Roy Soc Lond 308:433–613Google Scholar
  44. Sibly RM, Calow P (1986) Physiological Ecology of Animals: An Evolutionary Approach. Oxford: BlackwellGoogle Scholar
  45. Simmons MJ, Preston CR, Engels WR (1980) Pleiotropic effects on fitness of mutations affecting viability inDrosophila melanogaster. Genetics 94:467–475Google Scholar
  46. Smith RH, Sibly RM, Møller H (1987) Control of size and fecundity inPieris rapae: towards a theory of butterfly life cycles. J Anim Ecol 56:341–350Google Scholar
  47. Soliman MH (1982) Directional and stabilizing selection for development time and correlated response in reproductive fitness inTribolium castaneum. Theoret Appl Gen 63:111–116Google Scholar
  48. Turner JRG (1977) Butterfly mimicry: the genetical evolution of an adaptation. Evol Biol 10:163–206Google Scholar
  49. Turner JRG (1984) Mimicry: the unpalatability spectrum and its consequences. Pp 141–165in Vane-Wright RI & Ackery PR (eds) The Biology of Butterflies. Symp R Ent Soc No 11. London: Academic PressGoogle Scholar
  50. Tursch B, Daloze D, Dupont M, Hootele C, Kaisin M, Pasteels JM, Zimmermann D (1971) Coccinellin, the defensive alkaloid of the beetleCoccinella septempunctata. Chemia 25:307Google Scholar
  51. Tursch B, Daloze D, Braekman JC, Hootele C, Pasteels JM (1975) Chemical ecology of arthropods — X. The structure of myrrhine and the biosynthesis of coccinelline. Tetrahedron 31:1541–1543Google Scholar
  52. Tursch B, Braekman JC, Daloze D (1976) Arthropod alkaloids. Experientia 32:401–407Google Scholar
  53. Williams GC (1966) Natural selection, the costs of reproduction and a refinement of Lack's principle. Amer Nat 100:687–690Google Scholar

Copyright information

© Georg Thieme Verlag Stuttgart 1991

Authors and Affiliations

  • Graham J. Holloway
    • 1
  • Peter W. de Jong
    • 1
  • Paul M. Brakefield
    • 1
  • Helene de Vos
    • 1
  1. 1.Section of Evolutionary Biology, Department of Population BiologyUniversity of LeidenLeidenThe Netherlands

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